摘要
滨麦(Leymus mollis(Trin.)Pilger,NsNsXmXm,2n=28)具有抗旱、耐寒、抗多种真菌和细菌病害、茎秆粗壮、大穗多花等特性,是麦类作物品种改良的优异种质资源。本研究采用细胞遗传学方法对由八倍体小滨麦M842-16与硬粒小麦品种D 4286杂交F6代选育出的1个附加易位系DM 5911进行了鉴定和农艺性状分析。细胞学镜检显示,DM 5911的染色体构型为2n=44=22Ⅱ;根尖体细胞原位杂交鉴定显示,DM 5911含有2条完整的Ns染色体和2条易位的Ns染色体;花粉母细胞原位杂交鉴定显示,DM 5911的2条完整Ns染色体和2条易位Ns染色体可以进行正常的联会配对和遗传。表明DM 5911是1个遗传稳定、包含1对完整的Ns染色体和1对易位Ns染色体的小滨麦附加易位系。形态学调查显示,DM 5911在株高和分蘖数等农艺性状方面得到了明显的改善。该附加易位系作为进一步创制小滨麦小片段染色体易位系的重要种质材料,可用于小麦染色体工程育种与遗传改良。
Leymus mollis （Trin.） Pilger （NsNsXmXm, 2n = 28）, an excellent germplasm resource for wheat improvement, possesses many potentially valuable traits, such as strong stems, large spikes, multiple spikelets,tolerance of abiotic stresses and resistance to fungal and bacterial diseases. In this study,an addition and translocation line DM 5911 with the chromosome number of 2n=44 was devel- oped from the F6 progeny of octoploid Tritileymus M 842-16 × Triticum durum cv. D 4286 by using of cytogenetics and morphology. Screening of mitosis and meiosis showed that DM 5911 had a chro-mosome karyotype of 2n= 44= 22 Ⅱ. Mitosis GISH（genomic in situ hybridization） indicated that DM 5911 contains two whole Ns chromosomes and two translocation chromosomes between wheat and L. mollis Ns genome. Meiosis GISH indicated that the L. mollis Ns chromosomes perform normal synapsis,pairing and inheritance. The results suggested that DM 5911 is a cytogenetically stable wheat- L. mollis addition and translocation line, containing a pair of whole Ns chromosome and a pair of Ns translocated into wheat chromosomes. Morphology investigation showed that DM 5911 has improved in the agronomic traits,such as plant height and the number of tillers. This line can be used as a new germplasm for creating translocation line with small chromosome fragment in wheat chromosome engineering and genetic breeding.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2016年第8期996-1002,共7页
Journal of Triticeae Crops
基金
陕西省自然科学基金项目(2015JM3095)
西北农林科技大学唐仲英育种基金项目
关键词
小麦
滨麦
附加
易位
基因组原位杂交
Wheat
Leymus mollis
Addition
Translocation
Genomic in situ hybridization（GISH）
作者简介
第一作者E—mail：cxn622@126．com
通讯作者：赵继新（E-mail：zhjx881@163．com）
