摘要
目的通过观察外周血单个核细胞（ PBMCs）中miR-146a的表达水平与炎症因子肿瘤坏死因子α（TNF-α）、C反应蛋白（CRP）和白细胞介素6（IL-6）的关系,探讨miR-146在糖尿病周围神经病变（ DPN）发生发展中的可能作用机制。方法应用实时荧光定量PCR检测2型糖尿病37例、2型糖尿病合并DPN 44例及健康对照组34名受试者PBMCs miR-146a的表达水平,并与TNF-α、CRP、IL-6等临床指标进行相关性分析。结果与健康对照组、2型糖尿病不伴有DPN组相比,2型糖尿病伴有DPN组miR-146a的表达水平明显降低,而TNF-α、CRP、IL-6、HbA1C、收缩压、稳态模型评估的胰岛素抵抗指数（ HOMA-IR）明显升高（均P＆lt;0.05）；DPN组患者的糖尿病病程、血尿酸、血肌酐、尿微量白蛋白/肌酐比值明显高于2型糖尿病不伴有DPN组（均P＆lt;0.05）。 Pearson相关分析提示miR-146a的表达水平与病程、HOMA-IR、HbA1C、TNF-α、IL-6呈负相关。多元线性逐步回归结果显示HOMA-IR、HbA1C、TNF-α、IL-6为miR-146a的独立影响因素（均P＆lt;0.05）。结论 miR-146可能通过炎症反应、糖代谢、胰岛素抵抗参与了DPN的发生发展。
Objective To explore the possible role of miR-146a in the pathogenesis of diabetic peripheral neuropathy（ DPN） by investigating the correlation of miR-146a level in the peripheral blood mononuclear cells （PBMCs）with tumor necrosis factor α（TNF-α）, interleukin-6（IL-6）, and C reactive protein（CRP）. Methods The expression levels of miR-146a in the PBMCs were measured using realtime PCR in 81 patients and 34 healthy individuals. The correlation of miR-146a expression with clinical parameters was analyzed. Results Compared to those in the healthy individuals and patients without DPN, miR-146a level was significantly decreased in diabetic patients with DPN while TNF-α, CRP, IL-6, HbA1C, systolic blood pressure, and homeostasis model assessment of insulin resistance index（HOMA-IR） were significantly increased（all P〈0.05）. Duration of diabetes, blood uric acid, serum creatinine, and urinary albumin to creatinine ratio in diabetics with DPN were higher compared with diabetics without DPN（all P〈0. 05）. The expression level of miR-146a was negatively correlated with diabetes duration, HOMA-IR, HbA1C, TNF-α, and IL-6. Multiple linear regression showed that HOMA-IR, HbA1C, TNF-α, and IL-6 were independent factors of miR-146a. Conclusion miR-146a may be involved in the development of DPN through inflammation, glucose metabolism, and insulin resistance.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2016年第7期552-555,共4页
Chinese Journal of Endocrinology and Metabolism
