摘要
目的:分析不同来源海南裸花紫珠中毛蕊花糖苷和木犀草苷含量变化,为裸花紫珠药材质量控制提供参考。方法:采用HPLC法同时测定毛蕊花糖苷和木犀草苷含量,通过方差分析等方法比较不同产地、采收时间和储存时间的裸花紫珠成分含量差异。结果:29份不同来源海南裸花紫珠中毛蕊花糖苷和木犀草苷含量差异较大,其中毛蕊花糖苷变异系数为69.78%,木犀草苷变异系数为50.38%。毛蕊花糖苷含量受产地和储存时间影响显著(P<0.05),而木犀草苷则受产地因素影响显著(P<0.05)。9月到翌年1月,儋州裸花紫珠毛蕊花糖苷含量呈增加趋势,而木犀草苷变化较小。此外,本研究所建立的HPLC测定方法简便、快速、准确。结论:海南裸花紫珠药材成分含量变化较大,产地、采收时间和储存时间是影响药材质量的重要因素。
Objective： This study aims at analysing content variation of acteoside and luteoloside in Callicarpa nudiflora from Hainan province, which provides a reference for the C. nudiflora quantity control. Method： The content of acteoside and luteoloside were simultaneously determined by HPLC, and the ANOVA was used to analyze the content variance of 29 samples C. nudiflora from Hainan province in different origins, harvest time and storage time. Result： There were large differences between acteoside and luteoloside among the total 29 samples. The coefficients of variation （CVs） of acteoside and luteoloside content were 69. 78% and 50. 38%, respectively. The acteoside content was affected significantly by origin and storage time, and the luteoloside content was affected by origin significantly. The acteoside content presented a trend of increase from September to the following January in Danzhou, whereas the luteoloside content showed minor change. Moreover, the HPLC simultaneous determination method was simple, rapid and accurate. Conclusion： There were great variations of acteoside and luteoloside content in C. nudiflora from Hainan province, and origin. The harvest time and storage time were important factors for the C. nudiflora quantity.
出处
《中国现代中药》
CAS
2016年第8期996-1000,共5页
Modern Chinese Medicine
基金
海南省科技合作专项资金项目(KJHZ2015-15)
企业合作项目(PZS-JZT01)
作者简介
[通信作者]庞玉新，副研究员，研究方向：中药资源；Tel：（0898）23300268，E—mail：pyxmarx@126．com
