摘要
目的:使用毛细管电泳(CE)与二极管阵列检测(DAD)联用技术(CE-DAD)的差异检测方法,优化膀胱癌患者尿液特异性蛋白的检测条件。方法:对5例膀胱癌患者及5例健康志愿者尿液蛋白中的低丰度蛋白进行含量的差异检测,并使用电解质添加剂对原缓冲体系进行优化,对电渗流进行有效控制,提高低丰度蛋白的分离效率。结果:确立了使用毛细管电泳与二极管阵列检测联用技术对正常人组与非肌层浸润性膀胱癌患者组尿液蛋白的差异检测最佳条件,并经ELISA法平行试验,显示两组低丰度蛋白存在差异相关性。结论:对CE-DAD的差异检测条件进行了初步的探索,为未来标准化检测方法的建立奠定了基础。
Objective： To explore an optimized method for difference detection of urinary proteomie in patients with bladder cancer （BCs） by capillary electrophoresis separation technique coupled with diode array detector （CE-DAD）. Methods： For detecting the difference in the contents of each urinary low-abundance proteins between the two groups, electrolytic additives were used to improve the buffer system, and the concentration of electro-osmotic flow was well controlled in this condition, in order to increase the separation efficiency. Results： The optimal conditions were established to detect the difference between the bladder cancer and the control group by CE-DAD, and the difference and correlation were also tested by the parallel experiment of ELISA method. Conclusion： This paper makes a preliminary exploration on the condition of difference detection by CE-DAD, and establishes the foundation for the institution of standardized experimental method in the future.
出处
《天津医科大学学报》
2016年第4期359-363,共5页
Journal of Tianjin Medical University
关键词
毛细管电泳
尿液蛋白
差异检测
电渗流
capillary electrophoresis
urinary proteomic
difference detection
electro-osmotic flow
作者简介
作者简介姚远（1991-），男，硕士在读，研究方向：医学仪器
通信作者：高卫平。E-mail：gaoweiping@tijmu．edu．on。
