摘要
利用农杆菌介导法将Bt cry1Ah基因转入玉米自交系综31,获得对玉米螟有显著抗性的转基因玉米HGK60,为了研究其对鳞翅目害虫的杀虫活性,在室内和田间分别用亚洲玉米螟、棉铃虫和黏虫幼虫对HGK60玉米的杀虫效果进行检测。室内生物活性检测结果表明,取食HGK60玉米心叶的亚洲玉米螟幼虫在3 d后死亡率达100%;HGK60玉米对棉铃虫幼虫有毒杀作用,玉米不同部位对棉铃虫幼虫的杀虫效果存在差异;与非转基因玉米对照相比,取食HGK60玉米叶片一周后的黏虫幼虫体重增长明显受到抑制。田间生物活性检测结果表明,HGK60玉米对于亚洲玉米螟和棉铃虫有很强的杀虫活性,达到高抗级别,对于黏虫有一定的杀虫活性,为抗性级别。
Bt cry1Ah gene was transferred into inbred line maize Zong31 via Agrobacterium-mediated method,and transgenic maize HGK60 with significant resistance to corn borer was acquired. In order to investigate its insecticidal activity to Lepidoptera pests,we evaluated the insecticidal effects of HGK60 to Asian corn boner（Ostrinia furnacalis）,cotton bollworm（Mythimna separate（Walker））and oriental armyworm（Helicoverpa armigeraHubne）through laboratory and field bioassay. The results of laboratory bioassay indicated that no Asian corn boner feeding on HGK60 leaves survived. HGK60 presented the toxic effect on neonate of cotton bollworm,and different tissues of it had different insecticidal effects. Compared to the non-transgenic maize,the body-weight of armyworm neonate was significantly inhibited after a week of feeding HGK 60 leaves. The results of field bioassay showed that HGK60 had solid insecticidal effects to O. furnacalis and H. armigera in high resistance level,while the efficacy to M. separate was in resistance level.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第6期69-75,共7页
Biotechnology Bulletin
基金
转基因生物新品种培育重大专项(2014ZX08003-001)
关键词
cry1Ah基因
玉米
玉米螟
棉铃虫
黏虫
cry1Ah gene
maize
Ostrinia furnacalis
Helicoverpa armigera
Mythimna separate Hubne
作者简介
宋苗，女，硕士研究生，研究方向：植物分子生物学与基因工程；E-mail：songmia00202@163．com
通讯作者：郎志宏，女，研究员，研究方向：植物分子生物学与基因工程；E-mail：langzhihong@caas．cn
