摘要
建立一种乳样经简单预处理后采用超高效液相色谱-串联质谱同时检测4种重要的乳源低聚糖方法。牛乳及人乳经过脱脂、脱蛋白后经amide液相色谱柱用0.1%氨水-乙腈溶液梯度分离,采用质谱检测。2种唾液酸乳糖标准品的标准曲线在0.78~50.00 mg/L范围内线性良好(R^2>0.990),2种岩藻糖乳糖标准品的标准曲线在1.56~100.00 mg/L范围内线性良好(R^2>0.990)。加标回收率在80.00%~100.00%之间,重复性、精密度实验所得相对标准偏差均小于5.00%。
An ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） method was used to rapidly and simultaneously detect 4 oligosaccharides in bovine milk and human milk. Samples were defatted, deproteined, resolved on an ACQUITY BEH amide column using gradient elution with 0.1% ammonia water/acetonitrile as mobile phase, detected by mass spectrometric specific ion monitoring and quantified. The standard curves of 2 fucosyllactose oligosaccharides were linear within the range from 0.78 to 50.00 mg/L with R^2 0.990. The standard curves of 2 sialyllactose were linear within the range from 1.56 to 100.00 mg/L with R^2 0.990. The recoveries of spiked samples ranged from 80.00% to 100.00%. The relative standard deviation（RSD） of repeatability and precision was less than 5.00%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第14期86-91,共6页
Food Science
基金
"十二五"国家科技支撑计划项目(2013BAD18B04)
北京市科技计划项目(D141100004814002)
关键词
超高效液相色谱-串联质谱
低聚糖
牛乳
人乳
ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）
oligosaccharides
bovine milk
human milk
作者简介
魏京华（1989-）,女,硕士,研究方向为发酵乳制品.E-mail：weijinghua1989@163.com
通信作者：姜铁民（1971-）,女,教授级高级工程师,硕士,研究方向为乳品加工.E-mail：jiang515910@126.com
