摘要
背景:玉米醇溶蛋白具有优良的溶解性、耐热性及可降解性,但目前对其生物相容性及修复牙周炎缺损的效果尚存在较大争议。目的:分析玉米醇溶蛋白支架的生物相容性及其修复牙周缺损的效果。方法:采用溶剂浇铸/粒子沥滤法制备玉米醇溶蛋白支架。(1)体外实验:将人牙周膜细胞与玉米醇溶蛋白支架共培养18 d,扫描电镜观察细胞生长情况;(2)体内实验:取8只Beagle犬,制作牙周缺损模型,随机分为2组,实验组在缺损处置入玉米醇溶蛋白支架,对照组间断缝合牙龈组织。术后3个月,扫描电镜观察缺损部位。结果与结论:(1)体外实验结果:人牙周膜细胞在支架上附着良好,与支架贴附紧密,呈梭形,能围绕孔隙生长,表明玉米醇溶蛋白支架具有良好的细胞相容性;(2)体内实验结果:实验组支架完全溶解,骨小梁排列较规则,存在成熟的修复组织且与周围组织融合较好;对照组缺损部位基本愈合,骨纤维排列不规则,存在明显骨陷窝。实验组新生牙槽骨高度、骨缺损高度、结合上皮长度显著低于对照组(P<0.05),新生牙骨质显著高于对照组(P<0.05);(3)结果表明:玉米醇溶蛋白支架具有良好的细胞相容性,可促进牙周缺损的修复。
BACKGROUND： Zein has excel ent solubility, heat resistance and biodegradability, but its biocompatibility and effect on periodontal defects repair are under discussion. OBJECTIVE： To analyze biocompatibility of zein and its effect on periodontal defect repair. METHODS： Zein scaffold was prepared by solvent casting/particulate leaching. In vitro test： Human periodontal ligament cel s were co-cultured with zein scaffold for 18 days, and cel growth was observed by scanning electron microscope. In vivo test： Eight Beagle dogs were enrol ed to establish periodontal defect models, which were randomly assigned to receive zein scaffold implantation as experimental group, or interrupted suture as control group. Afterwards, the defect region was observed by scanning electron microscope at 3 months. RESULTS AND CONCLUSION： In vitro results： Human periodontal ligament cel s adhered wel and tightly on the scaffold with a fusiform, and could grow around pores. In vivo results： In the experimental group the scaffold dissolved completely, bone trabecular arranged regularly, and mature tissues appeared, to be integrated with the surrounding tissues; in the control group, the defect region almost healed, but there were irregular fibers and obvious lacunae. Moreover, compared with the control group, the height of new alveolar bone and bone defect, as wel as the length of junctional epithelium were significantly decreased, and new cementum was significantly increased in the experimental group （P 〈 0.05）. To conclude, zein scaffold has good biocompatibility and can promote periodontal defect repair.
出处
《中国组织工程研究》
CAS
北大核心
2016年第25期3726-3731,共6页
Chinese Journal of Tissue Engineering Research
基金
河北省中医药管理局科研计划项目(2014028)~~
作者简介
李敏，女，1979年生，河北省宁晋县人，主治医师，主要从事口腔临床及研究工作。
通讯作者：王瑶，河北医科大学第一医院口腔科，河北省石家庄市050030
