摘要
目的:探讨大鼠软骨细胞与滑膜间充质干细胞以1∶1比例接触式共培养,评价软骨细胞诱导干细胞在体外成软骨方向分化。方法:相同培养基体外培养大鼠滑膜间充质干细胞与软骨细胞,扩增至第3代滑膜间充质干细胞与第1代软骨细胞按1∶1比例混匀,以1×106/mL为终浓度的微团体外培养作为实验组,以相同终浓度细胞数的软骨细胞和滑膜间充质干细胞作为对照组,每组各接种6组。各组标本于体外培养21d后,通过形态学观察,组织学染色,RT-PCR检测产物等方法对其新生软骨进行评价。结果:实验组及对照组体外培养21d后,形成微团似软骨样组织,质地较韧,乳白色。免疫组化法进行Ⅱ型胶原鉴定,基质能被Ⅱ型胶原染色。RT-PCR结果显示诱导后的微团表达软骨特异性基因Ⅱ型胶原和蛋白聚糖。结论:滑膜间充质干细胞与软骨细胞通过接触式共培养形成较成熟的软骨。
Objective：To discuss direct co-culture of rat chondrocytes and synovial-derived mesenchymal stem cells at 1：1and evaluate chondrocytes induced differentiation of stem cells in vitro.Methods：Rat synovial-derived mesenchymal stem cells and chondrocytes were cultivated in vitro in the same medium.The 3rd generation of synovial-derived mesenchymal stem cells and the 1st generation of chondrocytes in the proportion of 1：1were mixed.The pellet at the final concentration of 1×106/mL was set as the experimental group,and the considerable population of chondrocytes and synovial-derived mesenchymal stem cells was set as the control group.After cultivated in vitro for 21 days,the newly-born cartilage was evaluated through morphological observation,histological stain,and RT-PCR detection products.Results：After cultivated for 21 days,pellet chondroid tissue was formed and presented tenacious texture and milk white.Type-II collagen was identified by immunohistochemical staining.Matrix could be stained by Type-II collagen.RT-PCR results indicated that pellet expressed cartilage specific genes Type-II collagen and proteoglycan.Conclusion：Through direct co-culture,synovial-derived mesenchymal stem cells and chondrocytes can form mature cartilage.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2016年第6期554-558,共5页
Journal of Oral Science Research
基金
国家自然科学基金项目(编号:31260229)
新疆维吾尔自治区优秀青年科技创新人才培养项目(编号:2014721046)
关键词
滑膜间充质干细胞
软骨细胞
共培养
组织工程
Synovial-derived mesenchymal stem cells
Chondrocytes
Co-culture
Tissue engineering
作者简介
邵博（1986～），男，新疆人，住院医师，硕士，主要从事软骨组织工程研究工作。
通讯作者龚忠诚，电话：13319822466
