摘要
目的了解2011年-2012年江西省儿童急性出血性结膜炎病原学特征。方法采用PCR和逆转录PCR(RTPCR)检测方法,对2011年-2012年江西省急性出血性结膜炎患儿结膜拭子标本同时进行肠道病毒70型、柯萨奇病毒24型变异株和腺病毒3种病毒核酸检测。结果 2011年-2012年江西省儿童流行性出血性结膜炎各月份均有发病,均为年初开始发病,且呈逐月增多,于5月-7月最高。2011年结膜拭子标本病毒核酸阳性率为77.78%。其中,腺病毒占98.10%,肠道病毒70型占1.90%;2012年结膜拭子标本中病毒核酸阳性率为70.45%,均为腺病毒。结论 2011年-2012年江西省儿童急性出血性结膜炎流行高峰为5月-7月,病原谱构成为腺病毒占病毒阳性的比率上升,肠道病毒比率则下降。
Objective To understand the pathogenic characteristics of children acute hemorrhagic conjunctivitis（ AHC） from 2011 to 2012 in Jiangxi. Methods The specimens of conjunctival swabs collected from the children patients with AHC during 2011-2012 in Jiangxi were collected for the virus nucleotide detection of enterovirus type 70,type 24 strains of coxsackie virus and adenovirus with PCR and RT-PCR methods. Results The epidemic children AHC happened in each month during 2011-2012,which all happened in the beginning of the year with an increasing trend,and reached the maximum during May-July in Jiangxi.The positive rate of conjunctival swab specimens for virus nucleic acid in 2011 was 77. 78%,among them,adenovirus accounted for 98. 10%,with HEV70 of 1. 90%; the positive rate of conjunctival swab specimens for virus nucleic acid in 2012 was 70. 45%,with all of them adenovirus. Conclusion The epidemic children AHC peak is in the 5-7 month during 2011-2012 in Jiangxi.The pathogenic spectrum structure shows that the positive ratio of adenovirus rise,while that of the enterovirus ratio decreases.
出处
《中国卫生检验杂志》
CAS
2016年第11期1630-1631,共2页
Chinese Journal of Health Laboratory Technology
基金
江西省卫生厅普通科技科研计划项目(20113116)
江西省教育厅科学技术研究项目(GJJ11356)
关键词
急性出血性结膜炎
腺病毒
肠道病毒70型
病原谱
Acute hemorrhagic conjunctivitis
Adenovirus
Human enterovirus 70
Pathogen spectrum
作者简介
龚甜（1982-），女，硕士，副主任技师，主要从事分子病毒学的研究工作。
