摘要
目的建立HPLC法同时测定八正颗粒中栀子苷、大黄素和大黄酚的含量。方法色谱柱：Agilent Eclipse XDB-C18（4.6 mm×250 mm,5μm）;柱温：25℃;流动相：甲醇-0.2%磷酸溶液,梯度洗脱;流速：1.0 ml·min^-1;检测波长：254nm;检测时间：30 min。结果栀子苷、大黄素和大黄酚的线性范围为分别为10.6~212、0.515~10.3、1.01~20.2μg·mL^-1。平均回收率分别为100.7%、101.4%、100.2%,RSD分别为0.82%、0.52%、1.29%（n=6）。结论本文方法操作简便、准确度高,能缩短八正颗粒样品的检测时长,提高栀子苷、大黄素和大黄酚含量测定结果的准确性。
Objective To establish an HPLC method for determining the content of gardenoside,emodin and chrysophanol in Bazheng granules. Methods Agilent Eclipse XDB-C18（ 4. 6 mm × 250 mm,5 μm） chromatographic column was used. The column temperature was 25 ℃. The mobile phase was methanol-0. 2% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min^-1and the detection wavelength was set at 254 nm. Results The linear range of gardenoside,emodin and chrysophanol was 10. 6- 212,0. 515- 10. 3 and 1. 01- 20. 2μg·mL^-1respectively. The average recovery was 100. 7%,101. 4% and 100. 2% respectively,and RSD was0. 82%,0. 52% and 1. 29%（ n = 6） respectively. Conclusion This method is simple,accurate and reliable,which may shorten the detection time of Bazheng granules and improve the determination accuracy of gardenoside,emodin and chrysophanol.
出处
《解放军药学学报》
CAS
CSCD
2016年第2期138-140,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
军队医疗机构制剂标准提高科研专项课题
No.14ZJZ20-3
关键词
HPLC
八正颗粒
栀子苷
大黄素
大黄酚
含量测定
HPLC
Bazheng granules
gardenoside
emodin
chrysophanol
content determination
作者简介
作者简介：黎春彤，博士，主管药师。研究方向：中药分析。Tel：（010）66867401；E—mail：chuntongli@Miyun．com
通讯作者：刘皈阳，E—mail：liuguiy@gmail．com
