摘要
利用覆盖玉米基因组的18对SSR引物,对270份贵州玉米地方种质资源及六大种质类群代表系进行多样性评价。结果显示：18对引物供检测102个等位基因,平均等位基因数5.7,变幅在2~10,平均多样性指数为0.53。根据SSR分子标记遗传相似系数矩阵,经中心化变换后,按类平均法（UPMGA）聚类分析,分为6大类,其中第Ⅱ大类和第Ⅳ大类又分为2个亚群,与贵州玉米地方种质资源的地理来源和系谱一致,六大种质类群代表系各自单独成类。根据聚类分析结果,以20%抽样比例,最大遗传距离方法构建的核心种质库能最大限度保存原种质库的遗传多样性。
18 pairs of SSR primers covering the genome of maize were used to detect 270 local germplasm of Guizhou. The results showed that： 18 pairs of primers detected 102 alleles,the average number of alleles is 5. 7,ranged from 2 to 10,the average diversity index is 0.53. According to the genetic similarity coefficient matrix,and after the center of the transformation,by the class average method（UPMGA）cluster analysis,is divided into six categories,of which the second and fourth category is further divided into two subgroups,which is unanimous to the geographical origin and genealogy,representatives six germplasm classed into separate category. According to cluster analysis,we use 20 % of the sampling ratio,maximum genetic distance method constructing the Core Collection to maximize genetic diversity preserved original genebanks.
出处
《西南农业学报》
CSCD
北大核心
2016年第5期1018-1022,共5页
Southwest China Journal of Agricultural Sciences
基金
贵州省科学技术基金"贵州玉米地方种质资源遗传多样性的分子评价及核心种质库构建"[黔科合J字(2012)2206]
贵州省高层次创新型人才培养对象项目(2014GZ60263)
作者简介
王春梅（1982-），女，助理研究员，硕士，从事玉米分子遗传育种，E-mail：hiehum2008@163．com，
通讯作者。
