摘要
为探究SNP标记与刺参(Apostichopus japonicus)性状之间的相关性,本研究运用高分辨率溶解曲线(HRM)分析方法,采用46个SNP标记对刺参家系的遗传多样性进行分析,运用一般线性模型对SNP标记与刺参体长、体宽、体重、体壁重和出肉率性状的关联性进行检测。结果表明,平均多态信息含量(PIC)的范围从0.0302到0.3750,观测杂合度(Ho)的范围是从0.0312到0.4688,18个位点显著偏离哈迪-温伯格平衡(P<0.05)。相关性分析显示,46个SNP位点中有10个位点与生长性状呈显著相关(P<0.05),其中位点SNP183的基因型AB与出肉率显著相关(P<0.05);位点SNP189的基因型AA与体长呈显著相关(P<0.05);8个SNP位点(SNP4、SNP146、SNP154、SNP163、SNP164、SNP175、SNP178和SNP216)的基因型BB与体长、体宽、体重、体壁重性状中的部分或全部呈显著相关,推断基因型BB可能是这些位点的优势基因型。
The aim of this study was to explore the correlation between SNP markers and growth traits of sea cucumber （Apostichopus japonicas. Forty-six SNP markers were used to analyze the genetic di- versity of a full-sib family with high resolution melting method, and examine the correlation of SNP markers with the body length, body width, body weight, body wall weight, dressing percentage with general linear model （GLM）. The results of genetic diversity showed that the polymorphism informa- tion content （PIC） value ranged from 0. 0302 to 0. 3750, and the observed heterozygosity ranged from 0. 0312 to 0. 4688. Significant departure from Hardy Weinberg equilibrium was observed at 18 of 46 SNPs （P〈0.05）. The analysis of correlation suggested that 10 of 46 SNPs correlated with the growth traits significantly （P〈0.05）. Of 10 SNP loci, the genotype of AB at SNP183 locus was correlated with dressing percentage while the genotype AA at SNP189 correlated with body length significantly. The genotype BB at eight loci （SNP4, SNP146, SNP154, SNP163, SNP164, SNP175, SNP178 and SNP216） significantly correlated with body length, body width, bodyweight, and body wall weight, respectively, suggesting that genotype BB was superiority at these loci.
出处
《海洋湖沼通报》
CSCD
北大核心
2016年第2期49-58,共10页
Transactions of Oceanology and Limnology
基金
国家科技支撑计划项目(2011BAD13B03)
国家海洋公益性行业科研专项(201305005)
教育部博士点基金(20130132110009)资助
关键词
刺参
SNP标记
相关性
生长性状
Apostichopus japonicas
SNP marker
growth trait
correlation analysis
作者简介
董玉（1990-），女，硕士，E-mail：dongyu14127@163．com通讯作者：李琪，Tel：0532-82031622；qili66@ouc．edu．cn
