摘要
为确定适用于多肽体外抗氧化活性的测定方法,以丁基羟基茴香醚（butyl hydroxylanisole,BHA）、VC和生育酚（vitamin E,VE）为对照,测定大豆肽的还原能力、螯合Fe2＋能力、清除2,2’-联氮双（3-乙基苯并噻唑啉-6-磺酸）二胺盐（2,2’-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt,ABTS）自由基和1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基及抑制脂质体氧化的活性.结果显示：在质量浓度为0～30 mg/mL时,大豆肽还原能力随质量浓度的增加而增强,与BHA、VE、VC类似,大豆肽最大还原能力仅为0.487.大豆肽抑制脂质体氧化的活性弱于BHA和VE,但具有较稳定的增加趋势,最大抑制率达到20.6%;在质量浓度为0～15 mg/mL时,大豆肽螯合Fe^2＋能力随质量浓度的增加而增强,最大螯合率为38.7%.然而,未检测出BHA、VE、VC具有螯合Fe^2＋的能力;在质量浓度为0～3.0 mg/mL时,大豆肽清除ABTS＋·活性与VC基本相同,大于BHA和VE,ABTS＋·清除率最大为61.2%.大豆肽DPPH自由基清除率最大仅为2.1%,清除DPPH自由基活性远低于VC（45.0%）、BHA（10.1%）和VE（10.7%）,表明螯合Fe^2＋能力、清除ABTS＋·及抑制脂质体氧化活性的测定方法适合用于评价大豆肽体外抗氧化活性.
Various methods including reducing power, Fe2+-chelating, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS) radical scavenging, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging and liposome oxidation inhibition capacity assays were used for evaluating the antioxidant activities of soybean peptide employing butyl hydroxylanisole(BHA), VC and VE as controls, aiming to investigate the applicability of these assays for measuring the antioxidant activities of polypeptide in vitro. The results showed that the reducing power of soybean peptide increased with its concentration in the range of 0–30 mg/m L, attaining a maximum value of only 0.487, and BHA, VC, and VE similarly exhibited reducing power in a concentration-dependent manner. The inhibitory activity of soybean peptide against liposome oxidation was lower than that of BHA and VE, and this antioxidant activity exhibited a stable upward trend with increasing concentration of soybean peptide, reaching maximum percentage inhibition of 20.6%. Fe2+-chelating capacity of soybean peptide increased up to 38.7% with its concentration in the range of 0–15 mg/m L, whereas BHA, VE and VC had no such activity. Soybean peptide in the concentration range of 0–3.0 mg/m L possessed ABTS radical scavenging activity very similar to that of VC and higher than that of BHA and VE, and the maximum scavenging percentage was 61.2%. However, the maximum DPPH radical scavenging percentage of soybean peptide was only 2.1%, which was much lower than that of VC(45.0%), BHA(10.1%), and VE(10.7%). Therefore, Fe2+-chelating capacity, ABTS radical scavenging, and liposome oxidation inhibition capacity assays can be used to investigate the antioxidant activity of soybean peptide in vitro.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第11期52-57,共6页
Food Science
基金
粮食公益性行业科研专项(201513006-3)
湖北省自然科学基金面上项目(2014CFB888)
武汉市国际合作项目(201231234466)
武汉轻工大学研究生创新基金项目(2014cx013)
关键词
多肽
体外抗氧化活性
测定方法
适用性
polypeptide
antioxidant activity in vitro
assay
applicability
作者简介
蔡俊（1989-）,男,硕士,研究方向为食品营养与安全.E-mail：cai7413@126.com
陈季旺（1970-）,男,教授,博士,研究方向为食品营养与安全.E-mail：jiwangchen1970@126.com
