摘要
目的采用纤维素酶-超声法从玉米须中提取槲皮素,得到最佳工艺,为进一步开发玉米须资源提供依据。方法以槲皮素得率为指标,通过单因素试验,研究乙醇体积分数、液料比、超声时间、超声功率及酶用量对槲皮素得率的影响,利用正交法对影响槲皮素得率的上述5个因素进行优化。结果最佳提取工艺参数为乙醇体积分数50%、液料比25:1(m L/g)、超声时间40 min、超声功率200 W、酶用量0.014 mg。在此条件下,通过3次验证实验,测得槲皮素的得率为(0.374±0.023)%。结论采用正交法优化纤维素酶-超声法提取玉米须槲皮素的工艺具有可行性。
Objective The cellulase-ultrasonic synergistic extraction conditions of quercetin from corn silk were optimized to improve the utilization of corn silk. Methods On the basis of single-factor tests, the extraction of quercetin was investigated with response to five variables, including ethanol concentration, liquid/solid ratio, ultrasonic time, ultrasonic power and enzyme dosage using orthogonal method. Results The optimal cellulase-ultrasonic synergistic extraction conditions for quercetin from corn silk were as follows： ethanol concentration was 50%, liquid/solid ratio was 25：1（m L/g）, ultrasonic time was 40 min, ultrasonic power was 200 W, and enzyme dosage was 0.014 mg. The yield of quercetin was（0.374±0.023）% under these conditions. Conclusion It is feasible to use orthogonal method to optimize the cellulase-ultrasonic synergistic extraction of quercetin in corn silk.
出处
《食品安全质量检测学报》
CAS
2016年第4期1667-1673,共7页
Journal of Food Safety and Quality
基金
吉林大学大学生创新创业训练项目(2015450533)~~
关键词
玉米须
槲皮素
超声波
纤维素酶
正交法
corn silk
quercetin
ultrasound
cellulase
orthogonal method
作者简介
田佳琦，主要研究方向为食品科学与工程。E—mail：2992840850@qq．com
通讯作者：徐艳阳，博士，副教授，主要研究方向为食品营养与安全。E-mail：xuyy@jlu．edu．cn
