摘要
本文通过同化枝腋芽丛生方式建立了盐生植物盐节木的快速繁殖体系。以在MS附加200 mmol·L-1 Na Cl的培养基上无菌种子萌发、生长共计4~5个月、高约3~4 cm的盐节木小苗为材料,从上截取0.5 cm长的同化枝小段为外植体,培养在MS附加不同浓度的6-BA与NAA的培养基中。结果表明：同化枝腋芽诱导与增殖最佳培养基均为MS＋0.05 mg·L-1 6-BA＋1mg·L-1 NAA或MS＋1 mg·L-1 6-BA,初代培养30 d后,腋芽诱导率达97.5%,平均芽增殖系数为2.50;继代增殖培养时,将同化枝切成1 cm长的小段芽增殖系数提高到4.92;一次性芽苗伸长与生根培养基为MS＋0.05 mg·L-1 IBA,生根率达99%;小苗经炼苗后移栽到沙子、营养土和蛭石体积比为1：1：1的基质中,成活率可达91%。
A rapid propagation system has been established by using assimilation branches via axillary bud cluster for halophyte Halocnemum strobilaceum. The seeds were sterile-germinated on MS medium supplemented with 200 mmol·L-1 Na Cl for 30 d, then seedlings were transplanted to new medium monthly. Assimilation branch segments about 0.5cm exited from 4- or 5-month-old seedlings were used as explants, and were cultured on MS medium supplemented with different concentrations of benzyladenine（6-BA） and naphthylacetic acid（NAA）. The result show the optimal medium for both axillary buds induction and proliferation was MS＋0.05 mg·L-1 6-BA＋1 mg·L-1 NAA or MS＋1 mg·L-1 6-BA. In these two media, axillary bud cluster induction rate reached to 97.5%, and multiplication coefficient of bud was about 2.50 after 30 d during initial culturing. In the subculture, assimilation branch segments, about 1.0 cm, were cut from initial culturing seedlings as explants, and the multiplication coefficient of bud reached to 4.92, especially cultured on MS＋1 mg·L-1 6-BA medium. Seedling elongation and rooting medium was MS＋0.05 mg·L-1 indole-3-butyricacid（IBA）, and rooting rate of seedlings was 99%. The survival rate of transplanted plantlets in medium contented of sand–vermiculite–nutrition soil（1：1：1, V/V/V） was 91%.
出处
《植物生理学报》
CAS
CSCD
北大核心
2016年第4期431-435,共5页
Plant Physiology Journal
基金
山西师范大学高等学校大学生创新性实验项目(SD-2011CXSY-9和SD2012CXSY-38)
山西师范大学生命学院项目(SMYKZ-28和SMYKZ-37)~~
关键词
盐节木
同化枝
腋芽
快速繁殖
Halocnemum strobilaceum
assimilating branch
axillary bud
rapid propagation
作者简介
通讯作者 E-mail：xhchen_0809@163.com）
