摘要
目的:探索白虎加桂枝汤对高尿酸血症并急性痛风性关节炎(HUA+AUG)治疗作用及抗炎机制。方法:雄性SD大鼠60只根据体质量随机分为:正常组、模型组、白虎加桂枝汤低剂量组(0.265g/100g),白虎加桂枝汤中剂量组(0.53g/100g),白虎加桂枝汤高剂量组(1.06g/100g),秋水仙碱组(0.05mg/100g)。连续给药7d,第4天构建急性痛风性关节炎模型。观察大鼠一般情况,检测大鼠血清UA、CRP、NLRP3、NF-κB、Caspase-1、TNF-α、IL-6、IL-1、IL-10指标,踝关节组织中NLRP3、Caspase-1、TNF-α、NF-κB蛋白的表达,观测关节肿胀度,进行步态观察,炎症指数评分及大鼠踝关节滑膜形态学观察。结果:白虎加桂枝汤可以减轻HUA+AUG大鼠模型关节肿胀度,改善步态及炎症评分,降低血清CPR,同时还能降低血清NLRP3、Caspase-1、NF-κB、TNF-α、IL-6、IL-1水平,升高血清中IL-10水平;降低踝关节组织中NLRP3、Caspase-1、TNF-α、NF-κB蛋白的表达。结论:白虎加桂枝汤具有治疗HUA+AUG的作用,其机制可能与抑制NLRP3炎性体激活,抑制炎症反应有关。
Objective:To explore the therapeutic effect and anti-inflammatory mechanism of Baihu Jiaguizhi Decoction on hyperuricemia and acute gouty arthritis(HUA+AUG).Methods:Sixty male Sprague-Dawley rats were randomly divided into normal group,model group,Baihu Jiaguizhi Decoction low dose group(0.265 g/100 g),Baihu Jiaguizhi Decoction medium dose group(0.53 g/100 g).Baihu Jiaguizhi Decoction high dose group(1.06 g/100 g)and colchicine group(0.05 mg/100 g).The acute gouty arthritis model was constructed on day 4 for 7 days of continuous administration.The general condition of rats was observed.To detected the serum UA,CRP,NLRP3,NF-κB,Caspase-1,TNF-α,IL-6,IL-1,IL-10 indicators,and the expression of NLRP3,Caspase-1,TNF-α,NF-κB protein in ankle joint tissue.To observe the degree of joint swelling,and the gait,inflammation index score and morphological observation of rat ankle synovium.Results:Baihu Jiaguizhi Decoction could reduce joint swelling in HUA+AUG rat model,improve gait and inflammation score,reduce serum CPR,and reduce serum NLRP3,Caspase-1,NF-κB,TNF-α,IL-6,IL-1 level,increase serum IL-10;reduce the expression of NLRP3,Caspase-1,TNF-α,NF-κB protein in ankle joint tissue.Conclusion:Baihu Jiaguizhi Decoction has the effect of treating HUA+AUG,and its mechanism may be related to inhibiting NLRP3 inflammasome activation and inhibiting inflammatory response.
作者
刘伟伟
刘秋玉
周子正
史丽强
甘静
杨雨竹
管鑫
高建东
LIU Wei-wei;LIU Qiu-yu;ZHOU Zi-zheng;SHI Li-qiang;GAN Jing;YANG Yu-zhu;GUAN Xin;GAO Jian-dong(Department of Nephrology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,TCM Institute of Kidney Disease,Shanghai University of Traditional Chinese Medicine,Key Laboratory of Liver and Kidney Diseases(Shanghai University of Traditional Chinese Medicine),Ministry of Education,Shanghai Key Laboratory of Traditional Chinese Clinical Medicine(14DZ2273200),Shanghai 201203,China;Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai 200082,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2019年第5期2254-2259,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
上海市2017年度"科技创新行动计划"临床医学领域项目指南(No.17401971400)~~
关键词
白虎加桂枝汤
高尿酸血症并急性痛风性关节炎
作用机制
抗炎作用
Baihu Jiaguizhi Decoction
Hyperuricemia and acute gouty arthritis
Therapeutic Efficiency
Anti-inflammatory mechanism
作者简介
通讯作者:高建东,上海浦东新区张衡路528号上海中医药大学附属曙光医院,邮编:201203,电话:021-20256528 E-mail:gaojiandong@hotmail.com
