摘要
目的:观察平喘颗粒对外泌体的分泌及miR-23b介导的气道平滑肌细胞增殖的影响。方法:通过构建哮喘小鼠模型并以空白小鼠为对照组,观察不同处理方式下小鼠肺泡灌洗液中外泌体的分泌及外泌体中miR-23b的表达;通过外泌体干预气道平滑肌细胞,分别检测平滑肌细胞的增殖、凋亡,及细胞中TβRⅡ蛋白的表达。结果:与空白组比较,模型组外泌体数量、标记蛋白Alix、TSG101、CD9、CD63的表达、平滑肌细胞的增殖及TβRⅡ蛋白的表达显著增加,miR-23b的表达和ASMCs的凋亡显著降低(P<0.01);与模型组比较,平喘颗粒显著降低外泌体数量、Alix、TSG101、CD9、CD63的表达、平滑肌细胞的增殖及TβRⅡ蛋白的表达,增加miR-23b的表达和ASMCs的凋亡(P<0.01)。结论:平喘颗粒能够促进外泌体中miR-23b的表达,进而下调ASMCs中TβRⅡ蛋白的表达,调控ASMCs增殖和凋亡,阻止哮喘气道重塑。
Objective:To study the effects of Pingchuan Granules on secretion of exosomes and airway smooth muscle cells proliferation by inhibiting miR-23 b.Methods:Asthmatic mice model was established and normal mice was used as control group.The secretion of exosomes in alveolar irrigating solution of asthmatic mice and the expression of miR-23 b in secretion of exosomes were observed under different treatments.Proliferation and apoptosis of airway smooth muscle cells were detected by CCK8 and flow cytometry,with the expression of TβRII protein was detect by Western Blot.Results:Compared with the control group,the number of exosomes,expression of Alix,TSG101,CD9,CD63 proteins,the proliferation of smooth muscle cells and the expression of TβRII protein in alveolar irrigating solution of the model group were significantly increased,and the expression of miR-23 b and the apoptosis of ASMCs were significantly decreased(P<0.01).Compared with the model group,the number of exosomes,the expression of Alix,TSG101,CD9,CD63 proteins,the proliferation of smooth muscle cells and the expression of TβRII protein in alveolar irrigating solution of the Pingchuang Granule group were significantly decreased,and the expression of miR-23 b and the apoptosis of ASMCs were significantly increased(P<0.01).Conclusion:Pingchuan Granules can promote the expression of miR-23 b in Exosome,further down-regulate the expression of TβRII protein in ASMCs,regulate proliferation and apoptosis of ASMCs,and prevent airway remodeling of asthma.
作者
袁星星
田春燕
师留杰
王婷
马建
李竹英
YUAN Xing-xing;TIAN Chun-yan;SHI Liu-jie;WANG Ting;MA Jian;LI Zhu-ying(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Nangang Branch of Heilongjiang Academy of Traditional Chinese medicine,Harbin 150001,China;First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Key Laboratory of Chinese Materia Medical,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2019年第5期2007-2011,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
黑龙江省自然科学基金面上项目(N o.H2017068)
哈尔滨市应用技术研究与开发项目(No.2017RAXXJ053)~~
作者简介
通讯作者:李竹英,黑龙江省哈尔滨市香坊区和平路24号黑龙江中医药大学附属第一医院呼吸科,邮编:150040电话:0451-82111401转6308,E-mail:lizhuying6808@126.com;通讯作者:建,黑龙江省哈尔滨市香坊区和平路24号黑龙江中医药大学附属第一医院内分泌科,邮编:150040,电话:0451-82111401转6938 E-mail:940184376@qq.com
