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湖北襄阳白车轴草根瘤菌耐盐性和耐酸碱性的初步研究 被引量:2

Salt,Acid and Alkali Resistance of White Clover (Trifolium Repens L.) Rhizobia in Xiangyang,Hubei
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摘要 为了获得高耐逆性的根瘤菌菌株,对来自湖北省襄阳市湖北文理学院4个不同位置与白车轴草共生的根瘤菌,分别采用划线法和液体培养法进行耐盐性和耐酸碱性研究。结果表明:其中3株根瘤菌在盐浓度最高为50 g/L的TY培养基上生长,有一株根瘤菌对于Na Cl具有很高的耐性,可以在80 g/L的Na Cl的TY培养基上正常生长。酸碱性试验表明,除其中一株在p H 9以上的固体培养基上不能生长外,其余3个根瘤菌株系在不同p H值的TY培养基上均生长良好。由此可见,与白车轴草共生的根瘤菌具有很强的耐盐和耐酸碱能力,为研发高耐逆的菌肥奠定了基础。 In order to obtain high resistance rhizobium strains,the salt resistance and acid and alkaliresistance of rhizobia symbiosis with white clover from 4 different locations at Hubei University of Arts andScience,Xiangyang City,Hubei Province,were studied using streaking method and liquid culture method.Theresults showed that the three stains of rhizobia could grow in TY medium with salt concentration up to 50 g/L,and one strain of rhizobium with high tolerance to Na Cl could grow normally in 80 g/L Na Cl TY medium.Thep H test showed that one strain could not grow in a solid medium above p H 9,the other three rhizobium strainscould grow well in TY medium of different p H values.Thus,the symbiotic rhizobia with white clover had strongresistance to salt,acid and alkali.The study laid a foundation for developing high-tolerance bacterial manure.
出处 《中国农学通报》 2016年第15期98-101,共4页 Chinese Agricultural Science Bulletin
基金 湖北文理学院大学生创新创业项目"隆中豆科植物根瘤菌抗逆性与应用研究"(201510519104)
关键词 白车轴草 根瘤菌 耐逆性 Na CL 酸碱 white clover rhizobia resistance Na Cl acid and alkali
作者简介 第一作者简介:王奎,男,1994年出生,湖北襄阳人,本科,研究方向:根瘤菌的耐逆性菌株的筛选。通信地址:441053湖北省襄阳市隆中路296号3#226,湖北文理学院化学工程与食品科学学院,E—mail:2207614538@qq.com。 通讯作者:赵慧君,1979年出生,湖北襄阳人,讲师,研究生,博士,研究方向:分子生物学与基因工程。通信地址:441053湖北省襄阳市隆中路296号3#119湖北文理学院化学工程与食品科学学院,E—mail:lamb1129@163.com。
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