摘要
目的检测血管内皮生长因子受体(EGFR)、BRAF及K-Ras在结直肠癌组织中的表达情况并探讨其在结直肠癌发病机制中的作用。方法采用免疫组织化学SP法检测136例结直肠癌及其相应癌旁正常结直肠组织中EGFR、BRAF和K-Ras基因的蛋白表达情况,并分析其与结直肠癌患者临床病理特征之间的关系。结果 136例结直肠癌组织中EGFR、BRAF及K-Ras蛋白均有表达,阳性率分别为70.59%(96/136)、50.74%(69/136)及47.48%(66/136),均高于其相应在癌旁正常结直肠组织中的表达阳性率〔分别为35.29%(48/136)、16.18%(22/136)及19.52%(27/136)〕,虽然K-Ras的蛋白表达阳性率在二者之间的差异无统计学意义(P>0.05),但EGFR和BRAF在结直肠癌组织中的蛋白表达阳性率均明显高于其在癌旁正常结直肠组织中的表达,差异有统计学意义(P<0.05)。EGFR表达与肿瘤的TNM分期、分化程度及淋巴结转移有关(P<0.05),BRAF表达与肿瘤的TNM分期有关(P<0.05),而K-Ras在结直肠癌组织中的蛋白表达与肿瘤的TNM分期、分化程度及淋巴结转移均无关(P>0.05)。相关性分析结果显示EGFR、BRAF及K-Ras基因的蛋白表达之间均无相关性(P>0.05)。结论 EGFR、BRAF过表达可能与结直肠癌的发生、发展有关,但K-Ras作用尚不明确,抗EGFR、BRAF靶向治疗对结直肠癌患者可能有益。
Objective To detect expressions of epidermal growth factor receptor（EGFR）, BRAF, and K-Ras genes in colorectal carcinoma tissues and explore pathogenesis in colorectal carcinoma. Method The expressions of EGFR, BRAF, and K-Ras genes were detected in these 136 colorectal carcinoma tissues and their corresponding adjacent normal colorectal tissues by immunohistochemistry. Results The expressions of EGFR and BRAF in the colorectal carcinoma tissues were significantly higher than those in their corresponding adjacent normal colorectal tissues（P〈0.05）, but the expression of K-Ras had no significant difference between these two tissues（P〉0.05）. The expression of EGFR gene was related to the TNM stage, lymphatic metastasis, or degree of differentiation. The expression of BRAF gene was related to the TNM stage. The expression of K-Ras gene wasn, t related to the TNM stage, lymphatic metastasis, or degree of differentiation. The correlation analysis results showed that there was no relation among the EGFR, K-Ras, or BRAF expression. Conclusions Up-regulated of EGFR and BRAF gene expressions might be related to development of colorectal carcinoma,and role of K-Ras is unclear. Anti EGFR and BRAF target therapy might be benefited for patients with colorectal carcinoma.
出处
《中国普外基础与临床杂志》
CAS
2016年第5期556-560,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
国家自然科学基金资助项目(编号:81172362
81101874)
社会发展科技攻关项目(编号:S2015YFSF0130
S2015YFSF0275)~~
作者简介
崔飞博（1981年-），男，陕西省西安市人，在职硕士研究生，主治医师，主要从事消化系肿瘤的基础与临床研究，E-mail：cuifeibo@sina．com
通信作者：孙学军，E-mail：sunxy@mail．xjtu．edu．cn
