摘要
建立了超高效液相色谱-串联质谱仪（UPLC-MS/MS）测定白酒中8种甜味剂（安赛蜜、糖精钠、甜蜜素、阿斯巴甜、三氯蔗糖、纽甜、阿力甜和甜菊糖苷）含量的分析方法。样品经蒸馏水稀释一定倍数后,过0.22μm微孔滤膜,直接进入UPLC-MS/MS中分析检测。Waters HSS T3色谱柱为分析柱,甲醇-0.1%乙酸水溶液为流动相进行梯度洗脱,在电喷雾电离源负离子模式下,采用多反应监测模式进行检测。结果表明,8种甜味剂在10~500μg/L的范围内线性关系良好（相关系数r〉0.996）,方法检出限在1~3μg/L之间,基质加标回收率在89%~106%之间,相对标准偏差≤9%,均可达到白酒中痕量甜味剂的检测要求。该方法前处理简单,测定快速、准确、灵敏度高,非常适合白酒中多种甜味剂的快速筛查和定量分析。
The method of ultra performance liquid chromatography-tandem mass spectrometric（UPLC-MS/MS） had been developed for simultaneous determination of eight sweeteners in Baijiu as follows： liquor samples were diluted with distilled water, then filtered through 0.22 μm microporous membrane, and LC separation was performed on a Waters Acquity UPLC system with a HSS T3 column, and 0.1 %（v/v） acetic acid-methanol solution were used as mobile phase for gradient elution, after LC separation, MS/MS was adopted for multiple reaction monitoring mode（MRM）. The calibration curves showed good linearity in the range of 10 μg/L to 500 μg/L with the correlation coefficients higher than0.996. The limits of detection（LODs） were between 1 μg/L and 3 μg/L. The matrix recoveries were within 89 %-106 % and the relative standard deviations（RSDs） were lower than 9%（n=6）. Such method had the advantages including simple pretreatment, fast and accurate measurement, and high sensitivity, which was suitable for qualitative and quantitative measurement of sweeteners in Baijiu.
出处
《酿酒科技》
2016年第5期107-109,114,共4页
Liquor-Making Science & Technology
关键词
超高效液相色谱-串联质谱仪
白酒
甜味剂
ultra performance liquid chromatography-tandem mass spectrometry
Baijiu
sweeteners
作者简介
柯润辉（1980-），博士，高级工程师，主要研究方向为食品安全分析与检测技术，E-mail：runhuike@163．com。
