摘要
为了优化鸡特异性CpG ODN活性分子体外筛选体系,避免核酸细胞毒性的干扰,增强细胞应答效果,尝试利用CEF代替鸡淋巴细胞进行CpG ODN活性分子的体外筛选。将设计的9条不同序列的CpG ODN分别刺激CEF,通过测定细胞增殖效果和相关免疫基因的表达变化,评判免疫应答效果是否准确反映了CpG ODN结构特征,确定CpG ODN最佳免疫刺激活性分子。结果显示,CEF对CpG ODN的刺激呈现出良好的免疫反应,正确体现了CpG ODN序列的构效关系,成功筛选到3条CpG ODN活性分子,表明CEF适合于CpG ODN免疫佐剂的体外筛选。
To avoid the interference of the cytotoxicity of nucleic acid and enhance the effects of immune re- sponse, CEF was used to screen chicken CpG ODNs in vitro instead of chicken lymphocytes.CEF were stimulated by nine designed CpG ODNs, respectively,then cell proliferation and fold changes in related immune gene expression were assayed,which was compared with the reaction of lymphocytes.As a result, strong immune responses were ex- hibited on CEF stimulated by CpG ODNs ,which was correlated with the structure of CpG ODNs.Finally,three CpG ODNs were selected for in yiyo test.Present study demonstrated that CEF was suitable for the screening of CpG ODNs in vitro.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2016年第5期649-655,共7页
Chinese Veterinary Science
基金
"十二五"农村领域国家科技计划项目(2015BAD12B03)
作者简介
刘伟杰（1989-），男，山东莱州人，硕士生
张琳（1984-），男，河南开封人，助理研究员，主要从事新型疫苗及免疫佐剂的研制，Tel：0531-88622611，E-mail：lzhang3406@163．com。
通信作者，教授．主要从事兽医微生物与免疫学研究
