摘要
【目的】研究北极海洋沉积物可培养细菌的菌种资源多样性。【方法】采用海水Zobell2216E培养基和涂布平板法对第六次北极科学考察获得的海洋沉积物开展细菌分离培养,通过16S rRNA基因系统发育分析了解可培养细菌的多样性。【结果】根据菌落形态特征,从40个站位的北极海洋沉积物样品中共分离并获得16S rRNA基因有效序列的细菌达445株;基于16S rRNA基因的相似性分析与系统发育研究结果表明,分离获得的细菌分属于细菌域的4个门、6个纲、13个目、28个科、49个属、91个种,其中γ-Proteobacteria占大多数;有12株与模式菌株的16S rRNA基因序列相似性小于97%,可能代表了6个潜在的细菌新物种;此次获得的细菌种类组成与以往第五次北极科考获得的相比,在属水平上差异较大。【结论】北极海洋沉积物中存在着丰富的微生物菌种资源,具有很多新型微生物仍未被发现,是亟待开发的微生物资源宝库。
[Objective] Investigating the diversity of the cultivable bacteria in Arctic Ocean sediments. [Methods] Culturable bacteria were isolated from marine sediments collected during the 6th Chinese National Arctic Expedition （Arc6） by marine Zobell 2216E medium and spread plate method. Subsequently, 16S rRNA gene sequence similarity analysis and phylogenetic analysis were performed. [Results] A total of 445 strains were isolated from 40 sediments samples. According tothe results of 16S rRNA gene sequence similarity as well as phylogenetic analysis, the isolates belong to 4 phyla, 6 classes, 13 orders, 28 families, 49 genera and 91 species. Twelve isolates having 16S rRNA gene similarity less than 97% with the closest type strain were grouped into 6 independent clusters, implying that these isolates might be six novel species. Our study also indicated that the genus composition of the strains from 6th Arctic Expedition were strikingly different with that from 5th Arctic Expedition （Arc5）. [Conclusion] There are abundant microbial resources and many novel microbes in Arctic Ocean sediments, and it is to be developed in the treasure house of microorganisms.
出处
《微生物学通报》
CAS
CSCD
北大核心
2016年第5期974-983,共10页
Microbiology China
基金
南北极环境综合考察与评估专项项目(No.CHINARE2015-03-05
CHINARE2015-04-03)
国家海洋局海洋生物活性物质与现代分析技术重点实验室资助项目(No.MBSMAT-2015-01)~~
关键词
北极海洋沉积物
可培养细菌
系统发育分析
多样性
： Arctic Ocean sediments, Culturable bacteria, Phylogenetic analysis, Diversity
作者简介
通讯作者：E-mail：linxz@fio．org．cn
