摘要
目的:探讨小剂量糖皮质激素对急性胰腺炎大鼠高迁移率族蛋白-1(HMGB-1)、髓样细胞触发受体-1(TREM-1)、肺组织髓过氧化物酶(MPO)、支气管肺泡灌洗液(BALF)的影响,为小剂量糖皮质激素临床治疗胰腺炎提供理论依据。方法:将72只SD大鼠随机分为3组,对照组(C组)、胰腺炎组(AP组)和胰腺炎+甲基强的松龙(APM组),5%牛磺胆酸钠逆行注入胰胆管建立大鼠急性胰腺炎模型,术后3、6、12、24h分别取样测定大鼠HMGB-1、TERM-1水平及MPO活性、BALF蛋白含量,并统计各组大鼠死亡率。结果:APM组与AP组相比,术后3、6、12、24hTREM-1mRNA水平较低(t=2.787、16.241、16.892、17.239,P均<0.05);与AP组相比,术后12、24hAPM组HMGB-1水平差异有统计学意义(t=12.231、15.125,P<0.01);与AP组相比,术后3、6、12、24hAPM组MPO活性均显著降低(t=2.148、16.103、16.476、17.219,P均<0.05);APM组BALF蛋白含量较AP组明显降低(P<0.01);APM组大鼠生存率较AP明显升高(χ2=7.271,P<0.01)。结论:小剂量的糖皮质激素能够降低与胰腺炎病情相关的TREM-1、HMGB-1、BALF、MPO等因子水平,有助于改善急性胰腺炎临床治疗效果,提高生存率,值得推广应用。
Objective：To evaluate the effect of small doses of glucocorticoids on HMGB-1,TERM-1,MPO and BALF in rats with acute pancreatitis,and provide theoretical basis for the clinical small doses of glucocorticoids treatment of pancreatitis.Methods：A total of 72 SD rats in the study were randomly divided into the control group（C group）,pancreatitis group（AP group）and pancreatitis＋ methylprednisolone（APM Group）,and 5% sodium taurocholate was retrograde injected into pancreaticobiliary duct to establish rat models with acute pancreatitis.A total of 3,6,12 and 24hafter operation,samples were collected respectively,HMGB-1levels,TERM-1levels,myeloperoxidase（MPO）activity and the protein contents of bronchoalveolar lavage fluids（BALF）were detected and death rates of all groups was counted.Results：mRNA levels of TREM-1of APM group were significantly lower than those of AP group,and the t-test results of 3,6,12 and 24hafter treatment were respectively（t=2.787,16.241,16.892,17.239,P〈0.05 for all）;compared with the AP group,HMGB-1levels of APM group at 12 and 24hwere significantly lower（t=12.231,15.125respectively;P〈0.01）;compared with the AP group,the MPO activity of APM group significantly decreased at all time phases of 3,6,12 and 24hafter treatment（t=2.148,16.103,16.476,and 17.219respectively;P〈0.05 for all）.Compared with AP group,BALF protein contents of APM group significantly decreased（P〈0.01）;compared with AP group,the survival rate of rats in APM group significantly increased（χ2=7.271,P〈0.01）.Conclusions：Small doses of glucocorticoids can reduce the factors associated with pancreatitis such as TREM-1,HMGB-1,BALF and MPO,it is good for improving clinical therapeutic efficacy and survival rate of AP,and it is worth popularization and application.
出处
《海南医学院学报》
CAS
2016年第13期1336-1338,1342,共4页
Journal of Hainan Medical University
基金
湖北省自然科学基金资助项目(c201823)~~
作者简介
牟来旭（1965-），男，土家族，本科，副主任医师，电话：15971775666，电子信箱：mulaixu@163．com。
