MiR-21在慢性阻塞性肺疾病患者外周血清和单个核细胞中的表达及临床意义被引量：9

Expression of miR-21 in peripheral blood serum and mononuclear cells in patients with chronic obstructive pulmonary disease and its clinical significance

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摘要目的:通过研究mi R-21在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者外周血清及单个核细胞中的表达,探讨mi R-21在COPD发病中的意义及机制。方法:将研究对象分为健康对照组(n=41)和COPD组(n=49),收集两组外周血清,分离其外周血单个核细胞并收集临床资料。运用实时荧光定量PCR法检测各组外周血清及单个核细胞中mi R-21的表达水平。分析COPD组mi R-21与肺功能的关系,并对mi R-21与COPD患者一秒用力呼气容积(forced expiratory volume in one second,FEV1)进行相关性分析。结果:COPD组外周血清及单个核细胞中mi R-21表达水平较健康对照组增高;COPD外周血清及单个核细胞中mi R-21差异表达与肺功能相关;COPD患者mi R-21表达水平与FEV1呈正相关。结论:COPD患者外周血清及单个核细胞中mi R-21表达升高可能与COPD发病过程及病情严重程度相关。 Objective： To explore expression of mi R-21 in peripheral blood serum and mononuclear cells of patients with chronic obstructive pulmonary disease（COPD）, and to discuss the signifi cance and underlying mechanisms.Methods： The subjects were divided into a healthy control group（n=41） and a COPD group（n=49）. The mi R-21 level was detected by quantitative real-time PCR. The correlations betweenmi R-21 and lung function or forced expiratory volume in one second（FEV1） were analyzed.Results： The expression levels of mi R-21 in the serum and mononuclear cells in the COPD group were significantly elevated compared with those in the healthy group（P〈0.05）. The expression of mi R-21 was correlated with the lung function of COPD patients. The expression level of mi R-21 in the COPD patients was positively correlated with FEV1. Conclusion： The upregulation of mi R-21 in peripheral blood serum and mononuclear cells of COPD patients may contribute to the pathogenesis of COPD and the severity of this disease.

作者谢丽华杨芳英孙圣华

机构地区中南大学湘雅三医院呼吸科长沙市第四医院呼吸科

出处《中南大学学报（医学版）》 CAS CSCD 北大核心 2016年第3期238-243,共6页 Journal of Central South University ：Medical Science

基金国家自然科学基金青年基金(81500033)~~

关键词微小RNA-21 慢性阻塞性肺疾病 Sma和Mad相关蛋白7 外周血单个核细胞 miRNA chronic obstructive pulmonary disease Sma-and Mad-related protein7 peripheral blood mononuclear cells

分类号 R563.9 [医药卫生—呼吸系统]

作者简介谢丽华，Email：xyelyhua@163．com 通信作者：孙圣华，Email：sunshenghua03@163．com

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