摘要
目的建立蒲黄中香蒲新苷和异鼠李素-3-O-新橙皮苷的含量测定方法。方法采用单因素考察方法建立供试品溶液制备方法;采用高效液相色谱法(HPLC)法测定香蒲新苷和异鼠李素-3-O-新橙皮苷的含量,色谱条件为Waters SunFire^(TM)C_(18)(4.6 mm×250 mm,5μm)色谱柱,乙腈-0.05%磷酸溶液梯度洗脱,流速1.0 m L/min,检测波长254 nm,柱温20℃。结果蒲黄药材采用80倍量的甲醇回流提取1.5 h,制备供试品溶液;香蒲新苷在0.245 2~1.471 0μg范围内线性关系良好,r=0.999 9,平均加样回收率为98.47%,相对标准偏差(RSD)=2.05%(n=6);异鼠李素-3-O-新橙皮苷在0.191 2~1.147 4μg范围内线性关系良好,r=0.999 7,平均加样回收率为100.56%,RSD=2.56%(n=6)。结论所建立的含量测定方法简便准确,重现性好,为蒲黄药材质量控制和评价奠定基础。
Objective To establish the determination method of typhaneoside and isorhamnetin-3-Oneohesperidoside in Pollen Typhae,Puhuang. Methods Single-factor test method was used to optimize the procedure of sample preparation. HPLC was used to detect the contents of typhaneoside and isorhamnetin-3-O-neohesperidoside in Pollen Typhae,Waters Sun Fire^TMC18（ 4. 6 mm × 250 mm,5μm） column at 20℃ and 254 nm detection wavelength,with mobile phase of acetonitrile-0. 05% phosphoric acid（ V / V） and flow rate of 1 m L / min. Results The sample solution was prepared by extracting from Pollen Typhae with 80 times amount of methanol for refluxing 1. 5 h. Typhaneoside showed a good linear relationship at the range of 0. 245 2 - 1. 471 0 μg（ r = 0. 999 9）,with the recovery of 98. 47%（ RSD = 2. 05%,n = 6）. Isorhamnetin-3-O-neohesperidoside showed a good linear relationship at the range of 0. 191 2 - 1. 147 4 μg（ r = 0. 999 7）,with the recovery of 100. 56%（ RSD = 2. 56%,n =6）. Conclusion The method established is simple,rapid,accurate and repeatable,which built up a good basis for the quality control and evaluation of Pollen Typhae.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2016年第4期304-307,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
北京中医药大学基本科研业务费资助项目(No.2013-ZDXKKF-23)
北京市教育委员会科技成果转化与产业化资助项目
作者简介
陈路晓，女，在读硕士生
通信作者：姜艳艳，女，博士，副教授，硕士生导师，主要研究方向：中药药效物质基础及质量控制方法研究，E-mail：jyyjm119@163．com
