摘要
适配体建立在仿生学基础上,是在体外从单链核酸随机序列库中筛选出的对某种靶物质具有高亲和度的寡聚核苷酸(DNA或RNA)片段。相较于食源性致病菌的常规检测手段,适配体具备特异性强、稳定性好及易于修饰等优点,因此在该领域得到了关注和应用。但前人对适配体与靶物质结合的原理认识尚不够深入,对食源性致病菌的适配体筛选及应用总结还存在许多不足。结合食源性致病菌的自身结构特点,介绍了该类物质的适配体筛选特点和检测领域中的最新研究进展,提出了目前存在的问题及发展趋势。
Aptamers are artificial DNA/RNA oligonucleotides binding target ligands with high affinities,and they are selected from the libraries of single-strand nucleic acid random sequence in vitro by SELEX. Compared with the traditional methods,application of aptamers has the obvious advantages of high specificity,chemical stability and simplicity of modification in the detection of food-borne pathogenic bacteria,thus it draws attentions and applications in this field. However,the combination principle of aptamers to the target has not been understood clearly yet,and summaries on the selection of aptamers against food-borne pathogenic bacteria and its application are still deficient. Combined with the characteristics of food-borne pathogenic bacteria,this article describes the selection characteristics of aptamers against them,and the latest progress in the detection field. Finally,the existing problems and development trend are proposed.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第4期48-62,共15页
Biotechnology Bulletin
基金
国家高技术研究发展计划("863"计划
2012AA101606)
北京市科技新星计划(XX2014B069)
关键词
适配体
食源性致病菌
传感器
检测
aptamer
food-borne pathogens
biosensor
detection
作者简介
解沛燕，女，硕士，研究方向：食品工程；E—mail：495366514@qq．com
通讯作者：许文涛，男，副教授，博士生导师，食品安全分子检测及分子毒理、转基因生物安全；E．mail：xuwentaollll@sina．com
