摘要
采用生物量测定、3,5-二硝基水杨酸比色法、对-棕榈酸硝基苯酯(ρ-NPP)比色法、福林-酚法等方法研究不同养殖密度和换水频率对蚂蟥生长、消化酶活性、生化指标和内在品质的影响。结果表明:蚂蟥的最终质量,特定生长率(SGR),增重率(WGR),淀粉酶,脂肪酶,蛋白酶活力,SOD,CAT,ALP酶活性与密度呈反比,与换水频率呈正比(P<0.05);换水频率与水中氨氮、亚硝酸盐和硫化氢呈负相关,与溶氧量呈正相关;养殖密度和换水频率对蚂蟥水分、总灰分、酸不溶性灰分、p H和抗凝血酶活性影响不显著。因此,每亩50万条为较适宜的养殖密度,每72 h 1次为较适宜的换水频率。
The effects of stocking density and exchanging water frequency on growth,digestive enzyme activity,anti-oxidative enzyme and inner quality of Whitmania pigra Whitman were evaluated with corresponding measures. The results showed that the eventual biomass,specific growth rate,gained weight rate,activities of amylase,lipase,protease,SOD,CAT,and ALP correlated positively with stocking density and negatively with exchanging water frequency（ P 0. 05）. Exchanging water frequency had negative correlation with ammonia nitrogen,nitrite,and hydrogen sulfide while revealed positive correlation with dissolved oxygen in the water. Stocking density and exchanging water frequency showed no significant effects on the contents of moisture,total ash,and acid-insoluble ash. It suggested that the optimum stocking density was 7. 5 million per hectare and the appropriate exchanging water interval was 72 h.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第6期995-1000,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(8147006)
南京农业大学大学生SRT项目(1415C18)
关键词
蚂蟥
养殖密度
换水频率
生长
消化酶
抗逆酶
Whitmania pigra
stocking density
exchanging water frequency
growth
digestive enzyme activity
anti-oxidative enzyme
作者简介
[通信作者]史红专，Tel：（025）84396591，E-mail：shihz@njau．edu．cn
