摘要
目的:研究细胞型朊蛋白(PrP^c)有无抑菌活性。方法:把Pr Pc分别与金黄色葡萄球菌、枯草芽孢杆菌、巨大芽孢杆菌、苏云金芽孢杆菌、大肠杆菌等5种菌孵育一定时间,然后用碘化丙锭对上述5种样品染色后采用流式细胞仪检测各样品的荧光信号。结果:通过对流式细胞仪的荧光信号进行分析,发现PrP^c对金黄色葡萄球菌和枯草芽孢杆菌具有明显的抑制活性,而另外3种菌的样品没有检测到荧光信号的增强,亦即PrP^c对这3种菌无抑菌活性。结论:细胞型朊蛋白有一定的抑菌活性,有望成为一种新型的抗菌肽。
Objective： To study the antibacterial activity of cellular prion protein（Pr Pc）. Methods： Incubated Pr Pc with the five randomly selected bacteria, Staphylococcus aureus, Bacillus subtilis, B.megaterium, B.thuringiensis and Escherichia coli BL-21 respectively for a period, then dyed the five sample with propidium iodide respectively fora certain time and detected the fluorescence signal of the five sample with flow cytometer ultimately. Results：The fluorescence signals of the five sample indicated that Pr Pchave visible antibacterial effect on S.aureus and B.subtilis, but enhanced fluorescence signals of the other three bacteria were not detected, so Pr Pchave no distinctantibacterial activity towards the other three bacteria. Conclusion： Cellular prion protein have a certain degree ofantibacterial activity, and it is expected to become a new type of antibacterial peptide.
出处
《生物技术通讯》
CAS
2016年第2期257-259,共3页
Letters in Biotechnology
关键词
细胞型朊蛋白
抑菌活性
抗菌肽
荧光信号
cellular prion protein
antibacterial activity
antimicrobial peptides
fluorescence signal
作者简介
王立娟（1981-），女，讲师，硕士,通信作者：王立娟，（E-mail）wlj．wang@163．com
