摘要
目的研究弗氏枸橼酸杆菌CF74的T6SS基因组岛功能。方法构建弗氏枸橼酸杆菌CF74的T6SS基因组岛的精确缺失突变株(CF74ΔT6SS)。对弗氏枸橼酸杆菌CF74和其突变株CF74ΔT6SS的全菌蛋白和上清蛋白进行提取,并进行2-DE分析。结果在CF74ΔT6SS全菌蛋白中,有4个蛋白表达上调,42个蛋白表达下调,并且Fli C的表达下调十分明显;在CF74ΔT6SS的培养上清中,有27个蛋白表达上调,36个蛋白表达下调,并且Fli C、Flg K和Fli D的表达下调十分明显。结论弗氏枸橼酸杆菌CF74的T6SS可以影响Fli C蛋白的表达和分泌。
Objective To screen fuction of CF74 T6 SS genomic island. Methods We constructed deletion mutant of T6 SS genomic island,and used 2-DE to compare the proteins in the WCL and culture supernatants from bacteria grown to stationary phase in LB between the wild-type and CF74ΔT6SS mutant. Results In WCL,4 and 42 protein-coding genes exhibited higher or lower levels of expression in CF74ΔT6SS,respectively. Fli C was found to have markedly decreased production in CF74ΔT6SS. In culture supernatants, we identified 27 up-regulated and 36 down-regulated proteins in CF74ΔT6SS. Three flagellar proteins,Fli C,Flg K and Fli D were identified,which were expressed less in CF74ΔT6SS.Conclusion The absence of T6 SS reduced the production and secretion of Fli C and at least 2 other flagellar proteins.
出处
《疾病监测》
CAS
2016年第3期185-191,共7页
Disease Surveillance
基金
国家自然科学基金(No.81301401)
传染病预防控制国家重点实验室面上项目(No.2011SKLID209)~~
作者简介
王艺婷，女，山西省阳泉市人，助理研究员，主要从事病原菌致病机理及生物信息学研究工作
通信作者：刘丽云，Tel：010-58900749，Email：liuliyun@icdc．cn
