摘要
目的探讨阿魏酸钠(SF)对心肌缺血再灌注(MI/R)损伤大鼠的保护作用及其机制。方法 40只SD大鼠随机分为假手术组、模型组、SF高剂量组(40 mg/kg)、SF低剂量组(20 mg/kg),每组10只。采用结扎左冠状动脉前降支30 min再灌注2 h的方法复制MI/R损伤大鼠模型,造模成功后取心脏测定心肌梗死面积、心肌组织细胞间黏附分子-1(ICAM-1)和核因子-κB(NF-κB)表达情况及髓过氧化物酶(MPO)的活力。结果 SF高、低剂量组心肌梗死面积小于模型组(P<0.05,P<0.01)。模型组心肌MPO活力和ICAM-1、NF-κB阳性表达率高于假手术组,SF高、低剂量组均低于模型组(P<0.05,P<0.01)。结论 SF对心肌MI/R损伤的保护作用是通过抑制中性粒细胞浸润,下调NF-κB和ICAM-1的表达,抑制炎性反应等途径实现的。
Objective To investigate the protective effect and mechanisms of Sodium Ferulate（ SF） on rats with myocardial ischemia/reperfusion（ MI/R） injury. Methods A total of 40 SD rats were randomly divided into sham operation group（ n = 10）,model group（ n = 10）,high-dose SF group（ 40 mg/kg,n = 10） and low-dose SF group（ 20 mg /kg,n = 10）. Rat models of MI/R injury was prepared by ligation of ramus descendens anterior arteriae coronariae sinistrae for 30 min followed by 2 h reperfusion. The hearts were obtained after successful model establishment,and the areas of myocardial infarction were calculated,and the intercellular cell adhesion molecule-1（ ICAM-1） and nuclear factor-κB（ NF-κB） expressions and myeloperoxidase（ MPO） activities in myocardial tissues were detected. Results The areas of myocardial infarction in high-dose and low-dose SF groups were less than that in the model group（ P〈0. 05,P〈0. 01）.The values of MPO activity,ICAM-1 and NF-κB positive rates in the model group were significantly higher than those in sham operation group,and the values in high-dose and low-dose SF groups were significantly lower than those in the model group（ P〈0. 05,P〈0. 01）. Conclusion The SF may protect MI/R injury by inhibiting neutrophil infiltration and inflammatory reaction and down regulating NF-κB and ICAM-1 expressions.
出处
《解放军医药杂志》
CAS
2016年第4期18-21,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
作者简介
通讯作者 李霞，E-mail：wjlixia0206@163．com
