摘要
为探究福建主栽的不同低温需求量砂梨Pyrus pyrifolia品种中休眠相关基因Pp MADS13-1和Pp MADS13-3序列及表达特征,以‘黄花’、‘蜜雪’两品种休眠时期的花芽为材料,克隆Pp MADS13-1和Pp MADS13-3基因,利用荧光定量PCR技术分析基因在休眠时期的表达变化情况。结果显示,Pp MADS13-1序列在砂梨两品种间较保守,而Pp MADS13-3序列在两品种间保守性较差;‘黄花’梨Pp MADS13-1hh和Pp MADS13-3hh在休眠期间随着休眠的解除下调表达,‘蜜雪’梨Pp MADS13-1mx和Pp MADS13-3mx在休眠期间呈现"M"型表达趋势。Pp MADS13-1和Pp MADS13-3均对砂梨的花芽休眠解除起到调控作用,在不同低温需求量品种间Pp MADS13-1和Pp MADS13-3的休眠调控模式有所不同。
The buds from ‘Huanghua＇ and ‘Mixue＇, two cultivars of Pyrus pyrifolia, were used as the experiment materials to explore the characteristics and expression of dormancy-associated MADS-box（DAM） genes Pp MADS13-1 and Pp MADS13-3 in different chilling requirement pear cultivars from Fujian. Pp MADS13 genes were cloned and used the QRT-PCR to analysis their expression changes during the dormancy period. The results showed that Pp MADS13-1 was conservative in low chilling requirement pears. The conservatism of Pp MADS13-3 was not so high in different chilling requirement pears. The Pp MADS13-1hh and Pp MADS13-3hh in ‘Huanghua＇ were gradual down-regulation with dormancy release. The Pp MADS13-1mx and Pp MADS13-3mx in ‘Mixue＇ presented a trend of ‘M＇ type during the dormancy. The result indicated that both Pp MADS13-1 and Pp MADS13-3 work in dormancy release, but their regulation model were different between two pear cultivars.
出处
《亚热带植物科学》
2016年第1期42-47,共6页
Subtropical Plant Science
基金
国家自然科学基金(31501694)
高等学校博士学科点专项科研基金(20113515110011)
作者简介
李亮，硕士研究生，从事果树分子生物学研究，E-mail：lihaimutang@163．com.
吴少华通讯作者。E-mail：wsh6677@hotmail．com；
李永裕通讯作者。E-mail：yongyu．li@fafu．edu．cn
