摘要
利用ISSR分子标记对202份海巴戟种质材料进行遗传多样性和聚类分析。结果表明:13条ISSR引物共扩增出65个位点,每个引物平均扩增出5个位点,其中多态性位点63个,占总数的96.92%。在相似系数为0.63时,202份海巴戟种质分为5个类,表现出一定的地域分布规律。检测到有效等位基因数Ne=1.604 2,基因多样性H=0.3 488,Shannon's信息指数I=0.520 6,说明202份海巴戟种质间存在着较高的遗传多样性。
The genetic diversity and cluster analysis of the 202 Morinda citrifolia germplasm resources were an- alyzed by using ISSR molecular markers. The results showed that 13 ISSR primers which amplified 65 loci, meaned the average of each primer could amplified five loci. Among them,63 bands (96.92%)were polymorphic. Cluster analysis result indicated that 202 germplasm resources could be clustered into five groups when the genetic similari- ty coefficient was 0.63, revealedthe characteristics of geographical distribution. The average of Shannon information index (I) , Nei's genetic diversity (H) and effective number of alleles (Nc) was 0. 5206, 0. 3488 and 1. 6042, respectively, which indicated there was highly genetic diversity in these 202 Morinda citrifolia resource .
出处
《西南林业大学学报(自然科学)》
CAS
北大核心
2016年第2期72-77,共6页
Journal of Southwest Forestry University:Natural Sciences
基金
国家自然资金项目(31360066)资助
关键词
海巴戟
ISSR
遗传多样性
聚类分析
Morinda citrifolia
ISSR
genetic diversity
cluster analysis
作者简介
廖丹(1989-),女,硕士生。研究方向:种质资源的保护和利用。Email:1015251405@qq.com。通信作者:符文英(1962-),女,硕士,教授。研究方向:作物遗传育种。Email:fwynoni@163.com。