摘要
目的研究汉黄芩素对人喉癌Hep-2细胞增殖、凋亡及侵袭能力的影响,并探讨其作用机制。方法运用CCK-8检测汉黄芩素对人喉癌Hep-2细胞增殖活性的影响。运用流式细胞仪检测汉黄芩素对人喉癌Hep-2细胞凋亡的影响。运用Hoechst染色法检测汉黄芩素对人喉癌Hep-2细胞核形态变化的影响。运用transwell侵袭实验比较汉黄芩素对人喉癌Hep-2细胞侵袭能力的影响。运用Western blot检测汉黄芩素对人喉癌Hep-2细胞h TERT、Bcl-2、Bax蛋白表达水平的影响。结果 CCK-8法发现汉黄芩素能抑制人喉癌Hep-2细胞增殖,其作用呈浓度依赖性。流式细胞术分析发现汉黄芩素能诱导人喉癌Hep-2细胞发生凋亡。Hoechst染色发现汉黄芩素能诱导人喉癌Hep-2细胞核形态变化。Transwell实验发现汉黄芩素能抑制人喉癌Hep-2细胞的体外侵袭能力。Western blot检测发现测汉黄芩素能有效降低人喉癌Hep-2细胞h TERT、Bcl-2蛋白表达水平以及增加Bax蛋白表达水平(P<0.05)。结论汉黄芩素在体外能够有效抑制人喉癌Hep-2细胞增殖,诱导细胞凋亡,同时抑制喉癌细胞的体外侵袭能力。其作用可能与调控Bcl-2蛋白家族及h TERT蛋白表达水平相关。
To investigate the effect and potential action mechanisms of wogonin on the proliferation,apoptosis and invasion in human laryngeal carcinoma cells（ Hep-2） in vitro. Methods After treated with different concentrations of wogonin,the effects of wogonin on proliferation activity of Hep-2 cells were detected by CCK-8,and its effects on cell apoptosis of Hep-2 were evaluated by flow cytometry. The effects of wogonin on nucleus morphologic change of Hep-2 were measured by Hoechst staining,and its effects on invasive ability of Hep-2 cells were detected by Transwell assay.Furthermore,the expression levels of h TERT,Bcl-2,Bax proteins were detected by Western Blot. Results The results by CCK-8 showed that wogonin could obviously inhibit proliferation of Hep-2,with a concentration- dependent manner. The results by flow cytometry indicated that wogonin could induce apoptosis of Hep-2. Hoechst staining suggested that wogonin could results in nucleus morphologic change of Hep-2,and Transwell assay showed that wogonin could inhibit the invasive ability of Hep-2. Moreover,Western Blot indicated that wogonin could obviously decrease the expression levels of h TERT and Bcl-2 and increase the expression levels Bax（ P〈0. 05）. Conclusion Wogonin can significantly inhibit cell proliferation,induced cell apoptosis and inhibit invasive ability of Hep-2 in vitro,and its action mechanism may be correlated to the regulation of expression levels of h TERT,Bcl-2 and Bax protein.
出处
《河北医药》
CAS
2016年第7期987-990,共4页
Hebei Medical Journal
作者简介
通讯作者：许磊；E-mail：12875710@qq．com
