摘要
目的:建立苗族药夜关门的薄层鉴别与含量测定方法。方法:采用薄层色谱法,以甲苯-甲酸乙酯-甲酸(10∶8∶1)为展开剂,建立了其定性鉴别方法;采用高效液相色谱法,以槲皮素为对照,Hypersil ODS2 C18色谱柱(4.6 mm×200 mm,5μm),流动相乙腈-0.20%磷酸溶液(20∶80),二极管矩阵检测器,建立了其含量测定方法。结果:建立的TLC能很好分离各成分,HPLC能够准确测定苗药夜关门中槲皮素的含量。结论:该文所建立的TLC,HPLC方法可用于苗药夜关门质量检测。
Objective： To establish methods of thin layer chromatography（ TLC） identification and content determination of Lespedeza cuneata. Method： Quality differentiation method was developed using TLC and with toluene-ethyl formate-formic acid（ 10 ∶ 8 ∶ 1） as the developing solvent. Using high performance liquid chromatography（ HPLC）,content determination of quercetin was carried on Hypersil ODS2 C18（ 4. 6 mm ×200 mm,5 μm） column eluted with a mixture of acetonitrile-0. 2% phosphate（ 20∶ 80） as the mobile phase,and diodematrix detector was used as the detector. Result： The established TLC method could effectively isolate each component,and the established HPLC method could accurately determine the content of quercetin in L cuneata.Conclusion： The established methods of TLC and HPLC can be used to detect the quality of L. cuneata.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第6期58-60,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技厅中药现代化项目(黔科合SY字[2014]3034-16号)
作者简介
[第一作者]刘亮，硕士，副教授，从事中药、民族药化学成分及质量研究，E-mail：jikmanl@163．com
[通讯作者]冯华，硕士，主管药师，从事药品检验及新药研究，E-mail：fenghua781014@163．com
