摘要
【目的】了解华北地区牛源无乳链球菌的生物学特性。【方法】在2012到2015年间从内蒙古自治区、河北、北京等地隐性乳房炎557份奶牛乳样中分离、收集无乳链球菌。采用纸片扩散法和PCR的方法对这些菌株分别进行耐药谱测定、荚膜分子分型、表面蛋白基因及毒力因子的检测。【结果】无乳链球菌的分离率为5.03%,其药物敏感性与其他地区无明显差别。分离到的28株无乳链球菌均属于荚膜Ia型,且毒力基因基本相同并且其表面蛋白均属于未定型。【结论】华北不同地区的无乳链球菌有相似的药物敏感性和毒力基因。为奶牛乳房炎无乳链球菌疫苗的研制及药物防治提供理论依据。
[Objective] In order to characterize Streptococcus agalactiae isolates of bovine origin from North China. [Methods] Five hundred and fifty-seven milk samples were collected in 2012-2015 from dairy cows with subclinical mastitis in Inner Mongolia Autonomous Region, Hebei Province and Beijing, and S. agalactiae isolates were identified by biochemical analysis and molecular biological methods. Their drug sensitivities were detected using standard disk difusion method, and the genes for capsular type, surface protein and virulence factors were amplified by PCR. [Results] Twenty-eight streptococcal isolates were ientified as S. agalactiae with an isolation rate of 5.03% and similar drug sensitivities. All of 28 S. agalactiae isolates belonged to capsular type Ia with similar virulence gene patterns and undefined surface protein types. [Conclusion] These data indicate that the S. agalactiae isolates of bovine origin in different regions of North China had similar drug sensitivities and virulence gene patterns, which provided the rationale for development of therapeutic strategy and vaccines against bovine mastitis S. agalactiae.
出处
《微生物学通报》
CAS
CSCD
北大核心
2016年第3期567-574,共8页
Microbiology China
基金
国家科技支撑计划项目(No.2012BAD12B09-02)~~
关键词
奶牛乳房炎
无乳链球菌
血清型
表面蛋白基因
毒力因子
Dairy cow mastitis
Streptococcus agalactiae
Serotype
Surface protein gene
Virulence factor
作者简介
通讯作者：E-mail：haoyq1960@163．com
