摘要
采用循环伏安法（CV）制备了曙红修饰玻碳电极（eosin Y/GCE）,电化学交流阻抗法对修饰电极表面进行了表征,研究了特丁基对苯二酚（TBHQ）在该修饰电极上的电化学行为,建立了循环伏安法和差分脉冲伏安法（DPV）测定TBHQ的新方法。研究表明,修饰电极对TBHQ的氧化还原具有较好的电催化活性,在eosin Y/GCE上的氧化还原峰电位差从297 mV降至85 mV。在20-400 mV·s^-1范围内,其氧化还原峰电流与扫速的平方根呈良好的线性关系,表明TBHQ在eosin Y/GCE上的电极反应受扩散控制。在0.10 mol·L^-1磷酸盐缓冲溶液（p H 6.5）中,扫速为100 mV·s^-1时,此修饰电极的DPV响应与TBHQ浓度在1-200μmol·L^-1范围内呈线性关系,检出限（S/N=3）为0.1μmol·L^-1。此修饰电极具有良好的选择性、重现性和稳定性,应用于油品中TBHQ的测定,回收率达95.0%-102.5%。该电极有望应用于多种食品中抗氧化剂的检测。
By using cyclic voltammetry,eosin Y film was electrodeposited on the surface of glassy carbon electrode（ GCE） to obtain the modified electrode（ denoted as eosin Y / GCE）. The electrochemical properties of eosin Y / GCE were studied by electrochemical impedance spectroscopy. The electrocatalysis capability of eosin Y / GCE to tertiary butyl hydroquinone（ TBHQ） was investigated by cyclic voltammetry（ CV） and differential pulse voltammetry（ DPV）. Compared with the bare GCE,eosin Y / GCE exhibited outstanding electrocatalytic activity and reversibility towards the oxidation of TBHQ. The oxidation and reduction peak separation was decreased from 297 mV to 85 mV for TBHQ at eosin Y / GCE. Influence of different scan rates（ 20- 400 mV·s^- 1） on redox of TBHQ was further examined at the eosin Y / GCE. The oxidation and reduction peak currents were linear to the square root of scan rate,indicating that the diffusion controlled process of TBHQ occurred at the eosin Y /GCE surface. Under the optimal conditions,the DPV response of the modified electrode was linear to TBHQ concentration in the range of 1- 200 μmol · L^- 1with a detection limit of 0. 1 μmol · L^- 1.Moreover,eosin Y / GCE exhibited excellent selectivity,reproducibility and long-term stability,and was successfully applied in the determination of TBHQ in soybean oil samples with recoveries of95. 0%- 102. 5%. This modified electrode could be used as a promising sensing platform for the determination of different antioxidants in food samples.
出处
《分析测试学报》
CAS
CSCD
北大核心
2016年第3期317-321,共5页
Journal of Instrumental Analysis
基金
国家级大学生创新创业训练计划(108792015002)
安徽科技学院重点学科(AKZDXK2015A01)
安徽科技学院自然科学研究项目(ZRC2014438)
关键词
曙红
特丁基对苯二酚
循环伏安法
差分脉冲伏安法
食品添加剂
eosin Y
tertiary butyl hydroquinone
cyclic voltammetry（CV）
differential pulse voltammetry（DPV）
food additives
作者简介
通讯作者：唐婧，硕士，讲师，研究方向：修饰电极和电分析化学，Tel：0550—2225981，E—mail：yayajing@shu．edu．cn
