摘要
目的:通过大蒜素预处理,观察全脑缺血再灌注大鼠海马区ICAM-1的表达,从而探讨大蒜素的脑保护机制。方法:雄性Wistar大鼠30只,随机分为5组:假手术组、缺血再灌注组、缺血再灌注+大蒜素10、20、30 mg/kg组。采用四血管闭塞法制备大鼠全脑缺血再灌注模型,于再灌注24 h取出海马,硫堇染色观察海马组织的形态学改变,免疫组织化学染色测定海马CA1区ICAM-1免疫反应阳性细胞面积和积分光密度值。结果:通过给予大鼠全脑缺血8 min再灌注24 h处理,海马CA1区组织形态学改变显著,神经元密度明显降低;ICAM-1的表达显著增加。静脉给予大蒜素可使缺血再灌注海马组织形态学改变明显改善,存活神经元数目增加,ICAM-1表达显著较少。结论:大蒜素可以通过减少ICAM-1的表达抑制全脑缺血再灌注后的炎症损失从而发挥脑保护作用。
Objective： Through the observation of allicin preconditioning, observing the expression on cerebral ischemia-reperfusion in ICAM-I area of hippocampus in rats, to study the mechanism of cerebral protection of allicin. Methods： 30 male Wistar rats, were randomly divided into 5 groups： sham operation group, ischemia reperfusion group, ischemia reperfusion＋garlicin 10, 20, 30 mg/kg group. By four vessel occlusion methods for the preparation of rats with global cerebral ischemia reperfusion model in 24 h after reperfusion, removing the hippocampus, thionine staining to observe morphological changes ofhippocampal tissue, Immunohistochemical staining was measured hippocampal CA1 area of ICAM-1 immunoreactivity and integral optical density of positive cells. Results： By giving complete cerebral ischemia 8min reperfusion in rats 24h, morphology of hippocampal CA 1 area organization changed significantly, neuron density decreased significantly; the expression of ICAM-1 increased significantly. Intravenous administration of allicin can make ischemia and morphology of hippocampal tissue perfusion changes significantly improved, the number of survival neurons increased, the expression of ICAM-1 was significantly less. Conclusion： Allicin can reduce ICAM-1 by inhibiting the expression and play a role in brain protection after cerebral ischemia - reperfusion inflammation loss.
出处
《现代生物医学进展》
CAS
2015年第35期6848-6851,共4页
Progress in Modern Biomedicine
基金
沧州市科学技术研究与发展指导计划项目(131302111)
作者简介
黄冬冬（1981-），女，硕士，主治医师，主要研究方向：神经内科，电话：18031792388，E-mail：luanma2006@sina.com
