摘要
以肌肉腐烂的黄颡鱼（Peheobagrusfulvidraco）为研究对象，从发病鱼体的肝、脾中分离出了一株致病菌（命名为JY-03），经革兰氏染色、显微观察、API生理生化实验24—48h，最终鉴定该病原菌为温和气单胞菌（Aeromonasso—bria）。通过感染实验再次证实黄颡鱼肌肉腐烂的致病菌为温和气单胞菌。对发病池塘水质分析表明，养殖水体中存在大量微囊藻，加速了水体中致病菌繁殖速度，致使发病池塘中黄颡鱼暴发肌肉腐烂病。药物的治疗实验表明，10％诺氟沙星与10％恩诺沙星分别与饲料按照1：1000（m／m）比例拌料，每日按照鱼体重1％投喂饲料1次，两种药物对该病均有明显治疗效果，鱼体皮肤溃烂征状逐步消失，死亡数量显著下降，疫病得到有效控制，用药2周后基本治愈；同等的用药剂量下，恩诺沙星治疗效果相对更好。本研究提供了黄颡养殖生产过程中肌肉腐烂病发病原因的鉴定方法和治疗方案，对黄颡鱼的健康养殖及相关研究均具有一定的参考意义。
The muscle decay of yellow catfish （ Pelteobagrus fulvidraco） was studied in this experiment. A strain of pathogenic bacteria （named JY -03 ） was isolated from the fish liver and spleen. The pathogenic bacteria was finally identified to be the Aeromonassobria by Gram staining, microscopy, API physiological and biochemical experiments. Furthermore, this bacteria caused yellow catfish muscle decay was also confirmed to be Aeromonassobria by the infec- tion experiment. The analysis of water quality showed that numerious microcystis accelerated pathogenic bacteria pro- liferation in aquacultural water, which caused muscle decay of Pelteobagru sfulvidraco in the incidence pond. The therapeutic trial was implemented using norfloxacin and enrofloxacin in fish tanks. 10% norfloxacin and 10% enro- floxacin were mixed separately with feed in accordance with 1：1000 scale, and the fish were fed once a day according to the 1% fish weight, It was found that both drugs had obvious therapeutic effects. The results showed that the symp- tom of skin decay disappeared gradually, the number of death was markedly decreased, and the spread of epidemic disease was prevented. The fish were almost cured after 2 weeks drugs treatment. Thereinto, enrofloxacin has a better effect than norfloxacin at the same dose. This study offered the identification and therapy method of muscle decay of Pelteobagrus fulvidraco in aquaculture process, and provided some references for healthy aquaculture of yellow catfish and other related research.
出处
《中国渔业质量与标准》
2016年第1期63-70,共8页
Chinese Fishery Quality and Standards
基金
2012年山东省级常规专家项目(370020120042)
作者简介
刘方（1983-），男，硕士研究生，工程师，研究方向为渔业病害防治和水产品安全分析，liufangl23f@163．com
通信作者：时彦民，研究员，研究方向为渔业病害防治和水资源及生态环境监测，shiyanmin000@163．com
