摘要
建立了测定水产品中MS-222残留的高效液相色谱一串联质谱（HPLC—MS／MS）分析方法。样品经甲醇和乙腈的混合液提取，再经二氯甲烷萃取后氮气吹干，用流动相溶解，经高效液相色谱分离，以串联质谱在多反应监测模式（MRM）下进行定性与定量分析。结果表明，在1．00、10．00和50．00μg/kg添加水平下，回收率81．2％-99．5％，相对标准偏差为2．14％-4．83％（n=6）。本方法对MS-222的定量限为1．0μg/kg。该方法前处理过程简便，容易操作，灵敏度和重现性良好，结果准确。
We established analysis method for the determination of MS - 222 residues in aquatic products by high per-formance liquid chromatography tandem mass spectrometry （HPLC -MS/MS） analysis method. The samples were ex- tracted by mixture of methanol and acetonitrile, followed by dichloromethane for drying with nitrogen, and was then dissolved in mobile phase. Through separation by the high performance liquid chromatography with tandem mass spec- trometry, qualitative and quantitative analysis was conducted under multiple reaction monitoring modes （MRM）. The results showed that the recovery rate was 81.2% -99.5% in 1.00, 10.00 and 50.00 μg/kg concentrations, and the relative standard deviation was 2.14% -4.83% （n = 6）. The limit of quantification was 1 μg/kg. The method of pretreatment process was simple, easy to operate, with sound sensitivity and reproducibility, and the results were ac- curate.
出处
《中国渔业质量与标准》
2016年第1期53-57,共5页
Chinese Fishery Quality and Standards
基金
国家水产品质量安全风险评估项目(GJFP2014009,GTFP201501003,GJFP201501004)
中央级公益性科研院所基本科研业务费专项资金项目(2014TS10)
作者简介
黎智广（1983-），男，研究实习员，研究方向为水产品安全与质量控制，lizhiguangl25@163．com
通信作者：黄珂，副研究员，研究方向为水产品质量安全研究及检测技术，xiamike@163．tom
