摘要
目的观察青蒿琥酯对大肠癌细胞的侵袭作用,并探讨其作用机制。方法采用不同浓度的青蒿琥酯(20、80、160μmol·L-1)作用于人大肠癌Lovo细胞,软琼脂集落形成实验检测青蒿琥酯对Lovo细胞锚着不依赖性增殖的影响;Transwell侵袭实验检测其对Lovo细胞侵袭的影响;Western blot方法分别检测Lovo细胞内HMGB1和MMP-2蛋白质水平的表达情况。结果青蒿琥酯能有效抑制Lovo细胞的增殖和侵袭能力,且呈剂量依赖性(P<0.01)。与对照组相比,不同浓度青蒿琥酯处理组HMGB1和MMP-2蛋白表达逐渐减少,差异有统计学意义(P<0.05)。结论青蒿琥酯可明显抑制大肠癌Lovo细胞侵袭,其机制可能与抑制HMGB1蛋白,下调MMP-2表达有关。
Aim To investigate the effect of artesunate on the invasion of human colon cancer Lovo cells and the possible mechanisms. Methods After Lovo cells were treated with different doses of artesunate( 20,80,160 μmol · L- 1),the soft agar colony formation test was adopted to observe the anchorage-independent proliferation of Lovo cells. Transwell assay was used to determine the effect of artesunate on the invasion ability of Lovo cells. And the protein expressions of HMGB1 and MMP-2 were investigated by western blot.Results Artesunate could significantly inhibit both proliferation and invasion ability of Lovo cells in a dose-dependent manner( P < 0. 01). The experimental group treated with artesunate significantly down-regulated the protein expressions of HMGB1 and MMP-2compared with control group( P < 0. 05). Conclusion Artesunate could inhibit the invasion of human colon cancer Lovo cells by down-regulating HMGB1 and MMP-2 expressions.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2016年第1期60-63,共4页
Chinese Pharmacological Bulletin
基金
河北省高等学校科学技术研究青年基金项目(No QN20131078)
河北省高等学校科学技术研究重点项目(No ZD20131004)
作者简介
郭颖（1982-）,女,硕士,讲师,研究方向：消化道肿瘤病理学,通讯作者,Tel：0313-4029293,E-mail：guoying-guo2008＠163.com
