摘要
目的:探讨新的脱细胞方法配合激光打孔制备的激光微孔脱细胞猪真皮支架对大鼠Ⅲ度皮肤烧伤创面血管化的影响。方法:将激光微孔脱细胞猪真皮(A组)、普通打孔脱细胞猪真皮(B组)两种真皮支架分别移植至Ⅲ度皮肤烧伤清创后创面,另设无真皮支架对照创面(C组),1周后加植表皮。大体观察植入真皮支架1、2、3周创面的修复情况,并通过HE染色和免疫组化标记创面中CD31、α-SMA表达阳性血管并计数。结果:制备的脱细胞猪真皮呈瓷白色,有光泽,柔软;显微镜下未见细胞残留。加植表皮后,A组较B组存活率高。两组支架植入后及无支架对照组1~3周创面中CD31、α-SMA表达阳性血管数持续增加,其中两组真皮支架均较无支架组多,差异有统计学意义(P<0.05);A组显著高于B、C组(P<0.05)。结论:该方法制备的激光微孔脱细胞猪真皮基质能快速血管化,提高表皮存活率,为一种较理想的真皮替代物。
Objective： To explore the influence of the new method-laser micropore porcine acellular demal matrix in vascularization of wounds in rats with full-thickness burn. Methods ： Laser micropore porcine acellular demal matrix （A group） and porcine acellular demal matrix （B group） were respectively transplanted on the rats with full-thickness burn after wound debridement, and there were no dermal in the control group （C group）. Then skin was transplanted into the rats of the three groups one week later. The reparation of dermal implant wound was recorded after 1,2 and 3 weeks respectively. At the same time, CD31, a-SMA positive signals detected by immunohistochemical staining and HE staining were used to observe the effect of repairation. Results：The well-prepared laser microspore porcine acellular dermal matrix was characterized by its porcelain white color, softness and good elasticity. Histological examination revealed that laser microspore porcine acellular dermal matrix was totally devoid of epidermis and cellular components. Group A had higher survival rate than group B. From lth to 3th week, the number of vessels with CD31 and cx-SMA expression were continuously increased in the three groups. The number of vessels with CD31 and c^-SMA expression in group A and B were significantly higher than in group C （P〈0.05）. The difference was statistically significant （P〈0.05）. Conclusion：Laser microspore porcine acellular demal matrix can be used as an ideal dermal substitute due to its ability of initiating revascularization rapidly which may increase the survival rate of the grafted skin.
出处
《天津医科大学学报》
2016年第1期17-20,共4页
Journal of Tianjin Medical University
关键词
真皮替代物
脱细胞真皮
猪
微孔
皮肤移植
烧伤
大鼠
dermal substitutes
acellular dermal matrix
microporous
skin transplantation
burns
rat
作者简介
盛小辉（1986-）。男，硕士在读，研究方向：吸入性损伤、重症烧伤、烧伤后整形；
通信作者：刘群，E-mail：zbcgzyx@163．com。
