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限制性末端片段长度多态性分析技术及其在肠道微生物研究中的应用 被引量：6

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摘要 限制性末端片段长度多态性分析技术(Terminal Restriction Fragment Length Polymorphism,T-RFLP)具有高通量,低成本,低劳动力等特点,是微生物生态学家在探索群落结构,功能及其动态变化中的一项实用方便的工具。自2012年至今,T-RFLP在肠道微生物领域研究方向不断拓宽,随后T-RFLP技术被更多的应用于人体相关疾病与肠道微生物关系的研究中。尽管T-RFLP技术仍存在不足,但随着16Sr RNA基因序列数据库和引物的改进,数据生成和分析统计工具的运用,T-RFLP最终将能在各个领域体现其价值,为人类疾病研究事业带来贡献。
作者 江欣倚 刘玲 熊靖飞 赵楚宁
机构地区 四川大学华西公共卫生学院
出处 《生物技术世界》 2016年第2期307-307,309,共2页 Biotech World
关键词 限制性末端片段长度 多态性分析 肠道菌群 动态变化 多样性
分类号 S831 [农业科学—畜牧学]
  • 相关文献

参考文献7

  • 1Osborn AM, Moore ERB, Timmis KN (1999) An evaluation ofterminal restriction fragment length polymorphism (T-RFLP)analysis for the study of microbial community dynamics. EnvironMicrobiol,2000(1):39-50.
  • 2孙庆华,柏耀辉,赵翠,温东辉,唐孝炎.DGGE、T-RFLP、LH-PCR对两种活性污泥的微生物种群多样性分析的比较[J].环境工程学报,2009,3(8):1365-1370. 被引量：31
  • 3Osborn AM, Moore ERB, Timmis KN (1999) An evaluation ofterminal restriction fragment length polymorphism (T-RFLP)analysis for the study of microbial community dynamics. EnvironMicrobiol,2000(1):39-50.
  • 4Akira Andoh,Toshio Kobayashi et al.;Characterization of gutmicrobiota profiles by disease activity in patients with Crohn'sdisease using data mining analysis of terminal restrictionfragment length polymorphisms;Biomedical Reports 2014(2):370-373.
  • 5Katsuyuki Fukuda,Yoshiyuki Fujita;Determination of the discriminantscore of intestinal microbiota as a biomarker ofdisease activity in patients with ulcerative colitis;BMC Gastroenterology2014(14):49.
  • 6Ricke P, Kolb S, Braker G Application of newly developed ARBsoftware- integrated tool for in silico terminal restrictionfragment length polymorphism analysis reveals the dominanceof a novel pmoA cluster in forest soil. Appl Environ Microbiol,2005(71):1671-1673.
  • 7Hugenholtz P, Goebel BM, Pace NR Impact of culture independentstudies on the emerging phylogenetic view of bacterialdiversity. J Bacteriol,1998(180):4765-4774.

二级参考文献21

  • 1余素林,吴晓磊,钱易.环境微生物群落分析的T-RFLP技术及其优化措施[J].应用与环境生物学报,2006,12(6):861-868. 被引量：67
  • 2Santegoeds C. M. , Ferdelman T. G. , Muyzer G. , et al. Structural and functional dynamics of sulfate-reducing populations in bacterial biofilms. Applied and Environmental Microbiology, 1998,64(10) :3731 - 3739.
  • 3OsbornA. M. , MooreE. R. B. , TimmisK. N. Anevaluation of terminal-restriction fragment length polymor- phism (T-RFLP) analysis for the study of microbial community structure and dynamics. Environmental Microbiology, 2000,2(1) :39 -50.
  • 4Raina V. , Suar M. , Singh A. , et al. Enhanced biodegradation of hexachlorocyclohexane (HCH) in contaminated soils via inoculation with Sphingobium indicum B90A. Biodegradation ,2008,19 ( 1 ) :27 - 40.
  • 5Mills D. K. , Entry J. A. , Gillevet P. M. , et al. Assessing microbial community diversity using amplicon length heterogeneity polymerase chain reaction. Soil Science Society of America Journal ,2007,71 (2) :572 - 578.
  • 6Ahn C. , Gillevet P. M. , Sikaroodi M. Molecular characterization of microbial communities in treatment microcosm wetlands as influenced by macrophytes and phosphorus loading. Ecological Indicators,2007,7 (4) :852 - 863.
  • 7Mills D. K. , Fitzgerald K. , Litchfield C. D. , et al. A comparison of DNA profiling techniques for monitoring nutrient impact on microbial community composition during bioremediation of petroleum-contaminated soils. Journal of Microbiological Methods ,2003,54 ( 1 ) :57 - 74.
  • 8Nielsen A. T. , Liu W. T. , Filipe C. , et al. Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor. Applied and Environmental Microbiology, 1999,65 ( 3 ) : 1251 - 1258.
  • 9Moeseneder M. M. , Arrieta J. M. , Muyzer G. ,et al. Optimization of terminal-restriction fragment length polymorphism analysis for complex marine bacterioplankton communities and comparison with denaturing gradient gel electrophoresis. Applied and Environmental Microbiology, 1999,65(8) :3518 -3525.
  • 10Marsh T. L. , Saxman P. , Cole J. , et al. Terminal restriction fragment length polymorphism analysis program, a web-based research tool for microbial community analysis. Applied and Environmental Microbiology, 2000,66 ( 8 ) : 3616 - 3620.

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生物技术世界
2016年 第2期

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