摘要
目的建立气相色谱-质谱法定量检测蔬菜中17种有机磷类混合农药残留的方法。方法样品经乙腈提取,氨基固相萃取小柱净化,浓缩,经GC-MS检测,选择离子扫描（SIM）模式监测目标化合物的特征离子进行定性定量分析。结果 17种化合物在检测的范围内线性关系良好（r〉0.99）,方法的检出限为0.001-0.020mg/kg（S/N=3）。在加标水平为0.04、0.08、0.40mg/kg时,方法的回收率为66.4%-99.3%,相对标准偏差为1.05%-6.81%。结论该方法特异度好、灵敏度高,适用于蔬菜中有机磷类农药的快速测定。
Objective To establish a method for quantitative determination of 17 kinds of organophosphates pesticides in vegeta- bles by gas chromatography and mass spectrometry（GC-MS）. Methods The sample was extracted by acetonitrile, purified by the a- mino solid phase extraction small column, concentrated, detected by GC-MS. Then the ion scanning （SIM） mode was selected to monitor the specific iron in target compound for conducting the quantitative analysis. Results 17 kinds o{ compound had good linearity in the limit of detection （LOD） of 0. 001-0.02 mg/kg （S/N=3） with a relative standard deviation of 1.05%-6.81%,and the recovery rate was 66.4%-99.3% when with cap at 004,008,0.40 mg/kg. Conclusion This method has good selection and high sensitivity,and is suitable for the rapid determination of organophosphates pesticide residues in vegetables.
出处
《国际检验医学杂志》
CAS
2016年第3期340-341,共2页
International Journal of Laboratory Medicine
基金
淮安市科技局科技发展计划基金项目(HAS2015030)
2015年度淮安市疾控系统预防医学科研课题项目(hayf201520)
关键词
气相色谱-质谱
固相萃取
蔬菜
有机磷
gas chromatography and mass spectrometry
solid phase extraction
vegetables
organophosphates pesticide
作者简介
宋鑫，男，主管药师，主要从事理化检验研究。
