摘要
为研究玉米秸秆和废弃白菜混合青贮可行性,考查二者在不同质量比时的混贮品质,设计了6个不同的混贮比例,分别为29∶19,27∶21,25∶23,23∶25,21∶27和19∶29。混合青贮30d后对其化学组分和发酵品质进行分析,筛选品质最佳的混贮比例,并进一步研究了品质最佳混贮组的乳酸菌多样性。结果表明,质量比为21∶27的混贮5(ME5)组的pH和氨态氮/总氮显著低于其余混贮组(P<0.05),乳酸含量显著高于其余混贮组(P<0.05)。ME5组的干物质和能源组分综纤维素含量较高,而酸性洗涤木质素含量较低,综合判定该组的混贮品质优于其他5组。乳酸菌多样性结果显示,从ME5组中共分离出10株乳酸菌,分属于3个属,4个种。3个属分别是乳杆菌属、肠球菌属和明串珠菌属。4个种分别是2株短乳杆菌、1株屎肠球菌、5株肠膜明串珠菌肠膜亚种和2株植物乳杆菌,其中同型发酵乳酸菌乳杆菌属和肠球菌属为该青贮体系的关键乳酸菌。
Silage made with a mixture of corn stalk(CS)and cabbage waste(CW)were mixed and ensilaged at different ratios,29∶19(ME1),27∶21(ME2),25∶23(ME3),23∶25(ME4),21∶27(ME5)and 19∶29(ME6),respectively.The chemical composition and fermentation traits were measured after 30days(d)to identify the optimal ratio of corn stalk and cabbage waste and the diversity of lactic acid bacteria in each mixture.The pH and the ratio of ammonia nitrogen to total nitrogen(AN/TN)of the ME5 silage was significantly lower than the other mixes(P<0.05).The lactic acid content(LA)of ME5 was significantly higher than the other mixes(P<0.05)whereas the ammonia nitrogen/total nitrogen ratio(AN/TN)of ME5 was significantly lower than other mixes.Analysis of lactic acid bacteria showed that a total of 10 lactic acid bacteria strains were isolated from the ME5 group,belonging to three different genera;Lactobacillus,Enterococcus and Leuconostoc.Two strains of Lactobacillusbrevis,one strain of Enterococcus faecium,five strains of Leuconostoc mesenteroides subsp.mesenteroides and two strains of Lactobacillus plantarum were identified.Among these isolates,Lactobacillus and Enterococcus were homofermentative lactic bacteria,which are able to produce more lactic acid than heterofermentative lactic bacteria.Lactobacillus and Enterococcus played a key role in the fermentation of these silages.In conclusion,mixed silages of CS and CW were found to be feasible with an optimum ratio of 21∶27respectively.
出处
《草业学报》
CSCD
北大核心
2016年第1期197-206,共10页
Acta Prataculturae Sinica
基金
国家自然科学基金(51366009)
国家高技术研究发展计划(863)(2014AA052801)
甘肃省自然科学基金(145RJZA064)
兰州市人才创新创业专项(2014-2-20)
兰州理工大学"红柳青年教师培养计划"(Q201207)资助
关键词
玉米秸秆
废弃白菜
混合青贮
乳酸菌
corn stalk
cabbage waste
mixed silage
lactic acid bacteria
作者简介
任海伟（1983-）,男,山西孝义人,副教授.E-mail：rhw52571119＠163.com
