摘要
以聚胸腺嘧啶寡核苷酸为模板分子，利用一步法温和快速合成了稳定性好、荧光性能优异的铜纳米粒子。基于L-半胱氨酸能通过巯基高选择性地与铜纳米粒子发生相互作用形成Cu—S键，从而淬灭铜纳米粒子荧光的现象，建立了测定L-半胱氨酸的荧光检测新方法。此方法测定L-半胱氨酸的线性范围为3～50μmol／L，检出限为0．56／μmol／L，可望用于尿液中L-半胱氨酸含量的测定。
A simple and sensitive method for the detection of L-cysteine was established using flu- orescent copper nanoparticles （CuNPs） synthesized by polythymine （poly T） as a templet. In the presence of L-cysteine, the fluorescence of CuNPs was tremendously quenched through a robust Cu-S bond between -SH in cysteine molecule and Cu of CuNPs. Under the optimized experimental conditions, the probe exhibited excellent performance in the range from 3 to 50 μmol/L with a de- tection limit of 0.56 μmol/L. Moreover, the developed bioassay was successfully applied to selec- tive detection and determination of L-cysteine in human urine samples.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第1期54-59,共6页
Journal of Shaanxi Normal University：Natural Science Edition
基金
国家自然科学基金(21275096)
作者简介
第一作者：吴佳，女，硕士研究生，研究方向为纳米材料的光学分析。E-mail：wujia19910113@163．com
通信作者：李保新，男，教授，博士生导师。E-mail：libaoxin@snnu．edu．cn
