摘要
根据云南松EST序列设计了32对SSR引物,从中随机选取7对引物,对不同群体云南松及细叶云南松、马尾松等近缘种进行了PCR,研究云南松EST-SSR引物在近缘物种中的通用性。结果表明:所选择的7对引物对云南松不同群体及其近缘种的扩增成功率均为100%;且7对引物在云南松不同群体的多态性比例均为100%,在细叶云南松、马尾松、高山松、油松和思茅松等近缘种中分别为71.43%、85.71%、85.71%、85.71%和100%;云南松EST-SSR引物具有较高的通用性,且这些引物可用于分析云南松及其近缘种间的遗传关系。
The transferability of Pinus yunnanensis EST- SSR markers to different populations of P. yun-nanensis and its related species was studied by 7 SSR primers randomly selected 32 SSR primers, which weredesigned based on ESTs. Polymerase chain reaction （PCR） to genomic DNA from P. yunnanensis,P, yun-nanensis var. tenuifolia, P. massoniana , P. densata, P. tabulaeformis and P. kesiya var. langbianensis weretested using 7 SSR primers. The amplification percentage of selected primers to different populations of P.yunnanensis and its related species were 100%. The percentage of polymorphism of 7 SSR primers was 100%of different populations in P. yunnanensis, and its related species were 71.43% , 85.71% , 85.71% ,85.71% and 100% , respectively. These results indicated that the P. yunnanensis EST - SSR marker washighly transferable to its related species, and these primers can be used to analyze the genetic relationship be-tween the P. yunnanensis and its related species. This study effectively proved that EST - SSR from P. yun-nanensis was valuable for genetic analysis and linkage mapping study among P. yunnanensis and its relatedspecies.
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2016年第1期16-20,共5页
Journal of Southwest Forestry University：Natural Sciences
基金
国家自然科学基金项目(31360189)资助
作者简介
第1作者：徐杨（1991-），男，硕士生。研究方向：林木遗传育种。Email：xuyang191@126.com。通信作者：许玉兰（1979--），女，博士，副教授。研究方向：林木遗传育种。Emml：xVYula@163.COm
