摘要
本研究旨在检测水牛STAT1基因的单核苷酸多态性(SNP),探究中国水牛多态性位点的群体遗传特征,寻找与产奶性状相关的分子标记。采用PCR和测序法筛选水牛STAT1基因序列的SNPs,以357头水牛为研究对象,采用基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF-MS)对群体进行了基因型检测,运用SAS(9.3)软件一般线性模型(GLM)对STAT1基因型与产奶性状的关联程度进行统计分析。结果表明,在水牛STAT1基因中检测出3个突变位点,分别位于5′UTR(g.68G>A)、外显子10(g.986G>T)和3′UTR(g.2881C>A)。关联分析结果显示,水牛STAT1基因g.986G>T位点与305天产奶量和乳脂率显著关联(P<0.05)。Bonferroni t检验多重比较结果显示,基因型TT和GG个体的305天产奶量显著高于GT基因型(P<0.05),基因型TT个体的乳脂率显著高于GG和GT基因型(P<0.05),且305天的产奶量随着胎次的增加呈现上升趋势,直到第3或4胎次时达到最大值,表明胎次是影响水牛产奶量的重要环境因素之一。本研究表明,水牛STAT1基因的g.986G>T位点可能是影响其产奶性状的候选分子遗传标记,为今后建立中国水牛标记辅助选择育种研究奠定基础。
This study aimed to identify single nucleotide polymorphism（SNP）of the buffalo STAT1 gene,investigate population genetics characters of SNPs and find molecular markers for milk production traits in Chinese buffaloes.The polymorphisms of STAT1 gene were detected by PCR and sequencing methods.Population genetic polymorphisms for 357 buffaloes were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology,and the associations analysis between genotypes and milk production traits was performed by the SAS（9.3）software with generalized linear model.The results showed that the total of 3SNPs were detected on the 5′UTR,exon10 and 3′UTR in STAT1 gene,namely g.68G〉A,g.986G〉T and g.2881C〉A,respectively.Association analysis indicated that 305-day milk yield and fat percentage were significantly associated with the g.986G〉T of STAT1gene（P〈0.05）.The results of Bonferroni t-test revealed that 305-day milk yield of individuals with TT and GG genotypes were significantly higher than those with GT genotypes（P〈0.05）,and fat percentage ofindividuals with TT genotype were significantly higher than those with GG and GT genotypes（P〈0.05）.And the 305-day milk yield tended to increase from first to later parities,reaching the maximum at the 3rd or 4th calving,indicating that parity was one of factors affecting the milk production of buffalo.Our findings in this study implied that the g.986G〉T of STAT1 gene might be a candidate molecular marker for milk production traits in buffalo,which provide a foundation for studying the marker-assisted selection programs in Chinese buffaloes.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2016年第1期48-54,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家国际科技合作专项(2014DFA31970)
桂渔牧科(1304512)
关键词
水牛
STAT1基因
产奶性状
飞行时间质谱
关联分析
buffalo
STAT1 gene
milk production traits
matrix-assisted laser desorption/ioniza tion time-of-flight mass spectrometry
association analysis
作者简介
邓廷贤（1983-），男，湖南郴州人，硕士，助理研究员，主要从事水牛分子遗传学研究，E—mail：dtx282000@163．com
通信作者：庞春英，副研究员，主要从事水牛遗传育种研究，E—mail：pangcy800@163．com
通信作者：梁贤威，研究员，主要从事水牛繁殖与营养研究，Email：liangbri@126．com；
