摘要
目的:优化白芍提取工艺。方法:以芍药苷含量为评价指标,以提取溶剂、溶剂用量、提取时间、提取次数为考察因素,采用混合均匀设计法结合二次多项式逐步回归方程制作曲面图,对白芍提取工艺进行优化并进行验证试验。结果:白芍的最优提取工艺为以90%乙醇为提取溶剂,溶剂用量为12倍,提取时间为150 min,提取2次;3次验证试验中芍药苷含量实测值为2.821 4%、2.795 7%、2.841 9%(RSD=0.82%,n=3),接近预测值2.848 4%。结论:本法简便、准确,建立的工艺可用于白芍的提取。
OBJECTIVE：To optimize the extraction technology of Paeonia lactiflora. METHODS：The surface figure made by mixed uniform design combined with the quadratic polynomial stepwise regression equation is adopted to optimize the extraction technology of P. lactiflora by using the content of paeoniflorin as index,with extraction solvent,the amount of extraction solvent,extraction time,extraction times as factors. RESULTS：The optimal extraction technology of P. lactiflora was as follows as 90%ethanol,12-folds extraction solvent,extracting for 150 min,extracting for 2 times. The measured value of 3 validation tests were2.848 4%,2.795 7%,2.841 9%（RSD=0.82%,n=3）,which was close to the predicted value 2.848 4%. CONCLUSIONS：The method is convenient and accurate,and can be used for the extraction of P. lactiflora.
出处
《中国药房》
CAS
北大核心
2016年第1期89-91,共3页
China Pharmacy
关键词
白芍
芍药苷
混合均匀设计
曲面图
提取工艺
Paeonia lactiflora
Paeoniflorin
Mixed uniform design
Surface figure
Extraction technology
作者简介
硕士研究生。研究方向：靶向制剂、药物新剂型与新技术。E—mail：yinxue0828@163．com
通信作者：主任药师，硕士生导师。研究方向：靶向制剂、药物新剂型与新技术。电话：0531—68617919。E—mail：tsunping@163．com
