摘要
目的探讨桦木酸对四氯化碳(CCl4)诱导雄性大鼠肝纤维化的治疗作用。方法通过改良经典CCl4造模法建立大鼠肝纤维化模型,给予大、中、小剂量(80、40和20mg/kg)桦木酸干预治疗,并以秋水仙碱(0.1mg/kg)作有效对照。治疗40d结束时,检测各组大鼠肝功能及关键性肝纤维化指标,光镜下比较各组肝脏组织病理改变。结果与空白组比较,各剂量桦木酸治疗组的血清ALT、AST、PC-Ⅲ、C-Ⅳ、LN和HA指标均有不同程度降低(均P<0.05),下降程度与桦木酸治疗浓度存在相关性,其中高剂量桦木酸治疗组下降最明显(P<0.01)。肝脏组织病理检查显示,各桦木酸治疗组大鼠肝纤维化有一定程度的改善。结论桦木酸具有抗肝纤维化的活性,在改善肝纤维化方面具有广阔的临床应用前景。该研究为进一步探讨桦木酸抗肝纤维化的功能及机制奠定了基础。
Objective To explore the treatment effect of betulinic acid on carbon tetrachloride-induced liver fibrosis in rats.Methods A modified model of carbon tetrachloride-induced hepatic fibrosis was established in rats,and betulinic acid was given in different dosages（80,40 and 20mg/kg）for 40 days with colchicine serving as control.The liver function and fibrosis indices were detected and the pathological changes of the liver tissues were compared between groups under the light microscope.Results The serum levels of ALT,AST,PC-Ⅲ,C-Ⅳ,LN and HA were decreased to different degrees in betulinic acidtreated groups compared with blank control group（P〈0.05 for all）.The decrease in these indices was related to the treatment concentration of betulinic acid,with the decrease being most significant in high-dose betulinic acid-treated group（P〈0.01）.Pathological examination of the liver showed that hepatic fibrosis was improved to some extent in all betulinic acid-treated groups.Conclusion Betulinic acid can protect against rat hepatic fibrosis,and it provides a broad clinical perspective on improving liver fibrosis.This study lays a foundation for further study on the function and mechanism of betulinic acid against liver fibrosis.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2015年第6期682-685,690,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
桦木酸
肝纤维化
四氯化碳
betulinic acid
liver fibrosis
carbon tetrachloride
作者简介
刘妙娜，女，1983年生，药学硕士，主管药师，E-mail：natalie627627@163．com
通讯作者，Correspondingauthor，E-mail：whzhchg@126．com
