摘要
为优化筛选最优树脂分离纯化根皮苷的工艺技术参数,以多穗石柯根皮苷提取液为原料,比较研究D101、X-5、ADS-7、S-8、AB-8、NKA-9、HPD-100及HPD-400 8种大孔吸附树脂对多穗石柯根皮苷的静态吸附与解吸效果。结果表明:S-8树脂对根皮苷有较好的吸附与解吸效果,静态吸附量与解吸量分别达到14.70 mg/g和8.395 mg/g。当主要考虑根皮苷得率时,最优工艺条件为根皮苷上样浓度0.9 mg/m L、上样流速3 BV/h、上样体积7 BV,洗脱剂乙醇体积分数60%、洗脱流速为4 BV/h、洗脱体积为3 BV,其根皮苷得率为89.89%,纯度为10.50%,根皮苷分离富集倍数为2.76倍;当主要考虑根皮苷纯度时,最优工艺参数为根皮苷上样浓度0.9 mg/m L、上样流速3 BV/h、上样体积7 BV,洗脱剂乙醇体积分数70%、洗脱流速为3 BV/h、洗脱体积为3.5 BV,其根皮苷纯度为13.51%,得率为83.26%,根皮苷分离富集倍数为3.56倍。
To optimize the technical parameters for separating phlorizin from Lithocarpus polystachyus Rehd.,phlorizin extracting solution of Lithocarpus polystachyus Rehd. was used as material, static adsorption anddesorption capacities of eight types of macroporous resin including D101, X-5, ADS-7, S-8, AB-8, NKA-9,HPD-100 and HPD-400 to phlorizin were analyzed. The results showed that S-8 had greater adsorption anddesorption capacities to phlorizin, which was 14.70 mg/g and 8.395 mg/g, respectively. When considering themaximum phlorizin yield, the optimal concentration, flow rate and volume of phlorizin solution was determinedto be 0.9 mg/m L, 3 BV/h and 7 BV, respectively; the optimal concentration, flow rate and volume of the eluentwas determined to be 60%, 4 BV/h and 3 BV, respectively, and the yield was 89.89%, which was 1.76 timesmore than the original solution, while the purity rate was 10.50%. Whereas when considering the maximumphlorizin purity, the optimal concentration, flow rate and volume of phlorizin solution was determined to be0.9 mg/m L, 3 BV/h, 7 BV, respectively; the optimal concentration, flow rate, and volume of the eluent was determined to be 70%, 3 BV/h and 3.5 BV, respectively, and the purity rate was 13.51%, which was 2.56 timesmore than the original solution, while the yield was 83.26%.
出处
《中国农学通报》
2015年第36期269-275,共7页
Chinese Agricultural Science Bulletin
基金
长沙市科技重点项目"多穗石柯根皮苷高纯品生产技术研究与开发"(K1401050-21)
湖南省科技项目"多穗石柯植物中根皮苷高效提制技术合作研究"(2013WK3002)
关键词
多穗石柯
根皮苷
大孔吸附树脂
分离纯化
Lithocarpus polystachyus Rehd.
phlorizin
macroporous resin
separation and purification
作者简介
秦昱，女，1991年出生，湖南长沙人，硕士，研究方向为茶及植物功能成分化学。林学院，Tel：0731—84673760，E—mail：qinyu199175@163．com。
通讯作者：肖文军，男，1969年出生，湖南城步人，教授，博士，研究方向为植物功能成分利用。林学院，Tel：0731-84673760，E-mail：xiaowenjun88@sina．com。通信地址：410128湖南省长沙市湖南农业大学园艺园通信地址：410128湖南省长沙市湖南农业大学园艺园
